Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.
Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.
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DOI：
10.1093/nar/gkr074
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发表时间：
2011-07
影响因子：
14.9
通讯作者：
Dieci G
中科院分区：
文献类型：
--
作者：
Orioli A;Pascali C;Quartararo J;Diebel KW;Praz V;Romascano D;Percudani R;van Dyk LF;Hernandez N;Teichmann M;Dieci G
Human RNA polymerase (Pol) III-transcribed genes are thought to share a simple termination signal constituted by four or more consecutive thymidine residues in the coding DNA strand, just downstream of the RNA 3′-end sequence. We found that a large set of human tRNA genes (tDNAs) do not display any T≥4 stretch within 50 bp of 3′-flanking region. In vitro analysis of tDNAs with a distanced T≥4 revealed the existence of non-canonical terminators resembling degenerate T≥5 elements, which ensure significant termination but at the same time allow for the production of Pol III read-through pre-tRNAs with unusually long 3′ trailers. A panel of such non-canonical signals was found to direct transcription termination of unusual Pol III-synthesized viral pre-miRNA transcripts in gammaherpesvirus 68-infected cells. Genome-wide location analysis revealed that human Pol III tends to trespass into the 3′-flanking regions of tDNAs, as expected from extensive terminator read-through. The widespread occurrence of partial termination suggests that the Pol III primary transcriptome in mammals is unexpectedly enriched in 3′-trailer sequences with the potential to contribute novel functional ncRNAs.
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通讯作者：
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