Improvement of mineral nutrient concentrations and pH control for the nitrite-dependent anaerobic methane oxidation process

Improvement of mineral nutrient concentrations and pH control for the nitrite-dependent anaerobic methane oxidation process
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亚硝酸盐厌氧甲烷氧化过程中矿物质营养浓度的提高和 pH 值控制

DOI:
10.1016/j.seppur.2016.02.016
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发表时间:
2016-04
影响因子:
8.6
通讯作者:
Baolan Hu
Baolan Hu
中科院分区:
工程技术1区
文献类型:
--
作者:
Zhanfei He;Sha Geng;Liqiao Wang;Chaoyang Cai;Jiaqi Wang;Jingqing Liu;Ping Zheng;Xinhua Xu;Baolan Hu

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A novel biological nitrogen removal process can be developed based on the newly discovered nitrite-dependent anaerobic methane oxidation (n-damo) process. In this work, the short- and long-term effects of mineral nutrients on the n-damo process were investigated by single-factor and orthogonal experiments, respectively. The pH buffering capacities of different media were determined by acid–base titration. KHCO3and KH2PO4greatly influenced the activity of n-damo bacteria and the pH changes of the media. An improved n-damo medium was proposed, containing 0.3 g L−1CaCl2, 0.2 g L−1MgSO4, 0.3 g L−1KH2PO4and 0.2 g L−1KHCO3. Moreover, the presence of 1% of CO2in gas could maintain the pH near neutral in the improved medium, which was first estimated in theory and then verified in practice. Finally, these results were applied in an n-damo reactor. The nitrogen removal rate was slightly increased (from 10.0 ± 1.2 to 10.8 ± 1.4 mg N L−1d−1), and the pH was quite stable after the improvement (daily changes from 0.58 ± 0.05 to 0.11 ± 0.02).
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发表时间: 2012
影响因子: 5.2
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