Carbon and nitrogen isotopic ratios of urine and faeces as novel nutritional biomarkers of meat and fish intake.
Carbon and nitrogen isotopic ratios of urine and faeces as novel nutritional biomarkers of meat and fish intake.
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DOI：
10.1007/s00394-012-0328-2
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发表时间：
2013-02
影响因子：
5
通讯作者：
O'Connell TC
中科院分区：
文献类型：
--
作者：
Kuhnle GG;Joosen AM;Kneale CJ;O'Connell TC
肉类和鱼类消费与慢性疾病风险的变化有关。摄入量主要通过自我报告进行评估，因为没有真正的定量营养生物标志物。血浆脂肪酸的测量，通常用作替代，是昂贵和耗时的。由于肉类和鱼类的稳定同位素比值不同，δ 13 C和δ 15 N已被提议作为生物标志物。然而，它们从未在对照人类饮食干预研究中进行过研究。在短期饲养研究中，我们研究了血液、尿液和粪便中δ 13 C和δ 15 N作为肉类和鱼类摄入量生物标志物的适用性。饮食干预研究（n = 14）遵循随机交叉设计，分为三个为期八天的饮食期（肉、鱼和半肉半鱼）。此外，4名参与者完成了素食控制期。在每个阶段结束时，收集24小时尿、空腹静脉血和粪便，分析其δ 13 C和δ 15 N。在粪便和尿液样品中，饮食之间的同位素比率存在显著差异，但在血液样品中没有显著差异（Kruskal-Wallis检验，p < 0.0001）。在成对比较中，与所有其他饮食相比，鱼类饮食后尿液和粪便样品中的δ 13 C和δ 15 N显著较高，素食后显著较低。对于血液、尿液或粪便样品，肉类和半肉半鱼饮食之间的同位素比率没有显著差异。本研究的结果表明，尿液和粪便中的δ 13 C和δ 15 N是短期肉类和鱼类摄入量的合适候选生物标志物。
Meat and fish consumption are associated with changes in the risk of chronic diseases. Intake is mainly assessed using self-reporting, as no true quantitative nutritional biomarker is available. The measurement of plasma fatty acids, often used as an alternative, is expensive and time-consuming. As meat and fish differ in their stable isotope ratios, δ13C and δ15N have been proposed as biomarkers. However, they have never been investigated in controlled human dietary intervention studies. In a short-term feeding study, we investigated the suitability of δ13C and δ15N in blood, urine and faeces as biomarkers of meat and fish intake. The dietary intervention study (n = 14) followed a randomised cross-over design with three eight-day dietary periods (meat, fish and half-meat–half-fish). In addition, 4 participants completed a vegetarian control period. At the end of each period, 24-h urine, fasting venous blood and faeces were collected and their δ13C and δ15N analysed. There was a significant difference between diets in isotope ratios in faeces and urine samples, but not in blood samples (Kruskal–Wallis test, p < 0.0001). In pairwise comparisons, δ13C and δ15N were significantly higher in urine and faecal samples following a fish diet when compared with all other diets, and significantly lower following a vegetarian diet. There was no significant difference in isotope ratio between meat and half-meat–half-fish diets for blood, urine or faecal samples. The results of this study show that urinary and faecal δ13C and δ15N are suitable candidate biomarkers for short-term meat and fish intake.
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影响因子：
4.7
作者：
Joosen AM;Kuhnle GG;Aspinall SM;Barrow TM;Lecommandeur E;Azqueta A;Collins AR;Bingham SA
通讯作者：
Bingham SA
DOI：
10.3758/bf03203630
发表时间：
1996-02-01
期刊：
BEHAVIOR RESEARCH METHODS INSTRUMENTS & COMPUTERS
影响因子：
--
作者：
Erdfelder, E;Faul, F;Buchner, A
通讯作者：
Buchner, A
影响因子：
2.7
作者：
del Rio, Carlos Martinez;Anderson-Sprecher, Richard
通讯作者：
Anderson-Sprecher, Richard
影响因子：
16.2
作者：
Patel, Pinal S.;Bingham, Sheila A.;Khaw, Kay-Tee
通讯作者：
Khaw, Kay-Tee
影响因子：
2.7
作者：
Joosen, Annemiek M. C. P.;Lecommandeur, Emmanuelle;Rodwell, Sheila A.
通讯作者：
Rodwell, Sheila A.