Organic Semiconductor Laser Platform for the Detection of DNA by AgNP Plasmonic Enhancement.
Organic Semiconductor Laser Platform for the Detection of DNA by AgNP Plasmonic Enhancement.
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用于通过 AgNP 等离子体增强检测 DNA 的有机半导体激光平台。
DOI：
10.1021/acs.langmuir.8b01313
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
McConnell G
中科院分区：
文献类型：
--
作者：
McConnell G
有机半导体激光器是一种灵敏的生物传感平台，可以响应特定的生物分子结合事件。到目前为止，这种生物传感器已经利用基于蛋白质的相互作用进行表面功能化，但是基于核酸的策略将大大拓宽它们作为通用生物诊断平台的实用性。这份手稿报告了两个重要的进展，DNA为基础的传感使用有机半导体（OS）分布反馈（DFB）激光器。首先，固定化的炔标记的12/18聚体寡脱氧核糖核苷酸（ODN）探针铜催化的叠氮化物炔环加成（CuAAC）或“点击化学”到80 nm厚的OS激光膜改性的叠氮化物呈递的单分子层。第二，检测与互补ODN-功能化银纳米颗粒的这些固定化探针的序列选择性结合。当结合发生时，纳米颗粒通过等离子体散射和吸收增加激光模式的光学损耗，并且这导致激光作用所需的阈值泵浦能量的上升，其与分析物浓度成比例。通过监测该阈值，实现了低至11.5pM的互补ODN靶标的检测。激光器表面上的这种互补结合通过表面增强拉曼光谱（SERS）被独立地证实。
Organic semiconductor lasers are a sensitive biosensing platform that respond to specific biomolecule binding events. So far, such biosensors have utilized protein-based interactions for surface functionalization but a nucleic acid–based strategy would considerably widen their utility as a general biodiagnostic platform. This manuscript reports two important advances for DNA-based sensing using an organic semiconductor (OS) distributed feedback (DFB) laser. First, the immobilization of alkyne-tagged 12/18-mer oligodeoxyribonucleotide (ODN) probes by Cu-catalyzed azide alkyne cycloaddition (CuAAC) or “click-chemistry” onto an 80 nm thick OS laser film modified with an azide-presenting polyelectrolyte monolayer is presented. Second, sequence-selective binding to these immobilized probes with complementary ODN-functionalized silver nanoparticles, is detected. As binding occurs, the nanoparticles increase the optical losses of the laser mode through plasmonic scattering and absorption, and this causes a rise in the threshold pump energy required for laser action that is proportional to the analyte concentration. By monitoring this threshold, detection of the complementary ODN target down to 11.5 pM is achieved. This complementary binding on the laser surface is independently confirmed through surface-enhanced Raman spectroscopy (SERS).
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