AutoSeqMan: batch assembly of contigs for Sanger sequences.
AutoSeqMan: batch assembly of contigs for Sanger sequences.
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AutoSeqMan:桑格序列重叠群的批量组装
DOI:
10.24272/j.issn.2095-8137.2018.027
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发表时间:
2018-03-18
影响因子:
4.9
通讯作者:
Sun YB
中科院分区:
文献类型:
--
作者:
Jin JQ;Sun YB
With the wide application of DNA sequencing technology, DNA sequences are increasingly generated through the Sanger sequencing platform. SeqMan (in the LaserGene package) is an excellent program with an easy-to-use graphical user interface (GUI) employed to assemble Sanger sequences into contigs. However, with increasing data size, larger sample sets and more sequenced loci make contig assemble complicated due to the considerable number of manual operations required to run SeqMan. Here, we present the ‘autoSeqMan’ software program, which can automatedly assemble contigs using SeqMan scripting language. There are two main modules available, namely, ‘Classification’ and ‘Assembly’. Classification first undertakes preprocessing work, whereas Assembly generates a SeqMan script to consecutively assemble contigs for the classified files. Through comparison with manual operation, we showed that autoSeqMan saved substantial time in the preprocessing and assembly of Sanger sequences. We hope this tool will be useful for those with large sample sets to analyze, but with little programming experience. It is freely available at .
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影响因子:
10.7
作者:
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者:
Tamura, Koichiro
影响因子:
7
作者:
Wall JD;Tang LF;Zerbe B;Kvale MN;Kwok PY;Schaefer C;Risch N
通讯作者:
Risch N
影响因子:
6.5
作者:
Yuan, Zhi-Yong;Zhou, Wei-Wei;Che, Jing
通讯作者:
Che, Jing
影响因子:
4.9
作者:
Sun YB
通讯作者:
Sun YB
DOI:
10.1073/pnas.74.12.5463
发表时间:
1977-01-01
影响因子:
11.1
作者:
SANGER, F;NICKLEN, S;COULSON, AR
通讯作者:
COULSON, AR