Graphene Substrates Promote the Differentiation of Inner Ear Lgr5+ Progenitor Cells Into Hair Cells.
Graphene Substrates Promote the Differentiation of Inner Ear Lgr5+ Progenitor Cells Into Hair Cells.
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石墨烯基底促进内耳 Lgr5 祖细胞分化为毛细胞
DOI：
10.3389/fbioe.2022.927248
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发表时间：
2022
影响因子：
5.7
通讯作者：
Huang, Zhichun
中科院分区：
文献类型：
--
作者：
Ding, Xiaoqiong;Hu, Yangnan;Cheng, Hong;Zhang, Xiaoli;Lu, Ling;Gao, Song;Cheng, Cheng;Wang, Lifen;Qian, Xiaoyun;Zhang, Chen;Chai, Renjie;Gao, Xia;Huang, Zhichun
治疗感音神经性耳聋的理想方法是通过干细胞疗法再生内耳毛细胞，从而恢复耳蜗组织的功能和结构。以前的研究发现，内耳中的Lgr5+支持细胞(SCs)可以再生HCs，因此被认为是内耳祖细胞。除了传统的生化因素外，电导率等物理因素在干细胞增殖和分化的调控中也起着至关重要的作用。本研究以石墨烯为底物，培养Lgr5+祖细胞，研究其对细胞的调控作用。结果表明，石墨烯基质对Lgr5+祖细胞具有良好的细胞相容性，并促进了其成球能力。此外，与组织培养聚苯乙烯(TCPS)相比，石墨烯基质上发现了更多的Myosin7a+细胞。这些结果表明，石墨烯是促进Lgr5+祖细胞分化为HCS的有效界面，这对其与Lgr5+细胞联合在内耳再生HCS具有重要意义。
The ideal treatment for sensory hearing loss is to regenerate inner ear hair cells (HCs) through stem cell therapy, thereby restoring the function and structure of the cochlea. Previous studies have found that Lgr5+ supporting cells (SCs) in the inner ear can regenerate HCs, thus being considered inner ear progenitor cells. In addition to traditional biochemical factors, physical factors such as electrical conductivity also play a crucial role in the regulation of stem cell proliferation and differentiation. In this study, the graphene substrates were used to culture Lgr5+ progenitor cells and investigated their regulatory effects on cells. It was demonstrated that the graphene substrates displayed great cytocompatibility for Lgr5+ progenitors and promoted their sphere-forming ability. Moreover, more Myosin7a+ cells were found on the graphene substrates compared with tissue culture polystyrene (TCPS). These results suggest that graphene is an efficient interface that can promote the differentiation of Lgr5+ progenitors into HCs, which is great significance for its future application in combination with Lgr5+ cells to regenerate HCs in the inner ear.
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DOI：
10.1073/pnas.1219952110
发表时间：
2013-08-20
影响因子：
11.1
作者：
Shi, Fuxin;Hua, Lingxiang;Edge, Albert S. B.
通讯作者：
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DOI：
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影响因子：
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29.4
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