Pseudomonas aeruginosa possesses three distinct systems for sensing and using the host molecule heme.
Pseudomonas aeruginosa possesses three distinct systems for sensing and using the host molecule heme.
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铜绿假单胞菌拥有三种不同的系统来感知和使用宿主分子血红素。
DOI：
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发表时间：
2019
影响因子：
5.1
通讯作者：
M. Llamas
中科院分区：
文献类型：
--
作者：
Joaquín R. Otero;A. I. García;Cristina Civantos;J. Quesada;M. Llamas
病原体已经开发了几种策略来在感染期间获得铁，包括使用来自宿主的含铁分子。血红素占脊椎动物铁库的绝大多数，因此是病原体铁的重要来源。使用蛋白质组学的方法，我们已经确定在这项工作中以前未表征的系统，我们命名为HXU，连同已知的具有和Phu系统，是由人类病原体铜绿假单胞菌响应血红素。我们表明，HAS和HXU系统是功能性的信号转导途径的细胞表面信号类和报告的机制触发这些信号系统的激活。两个信号级联都涉及外膜受体（分别为HasR和HxuA），其在感测细胞外介质中的血红素时在胞质溶胶中产生σECF因子的活化。HxuA在信号传导中起主要作用，在存在HasR和HexaR受体或其他铁源的条件下在血红素获得中起次要作用。值得注意的是，铜绿假单胞菌通过增加HxuA的产生来补偿HasR受体的缺乏，这强调了血红素信号传导对这种病原体的重要性。本文受版权保护。All rights reserved.
Pathogens have developed several strategies to obtain iron during infection, including the use of iron-containing molecules from the host. Heme accounts for the vast majority of the iron pool in vertebrates and thus represents an important source of iron for pathogens. Using a proteomic approach, we have identified in this work a previously uncharacterized system, which we name Hxu, that together with the known Has and Phu systems, is used by the human pathogen Pseudomonas aeruginosa to respond to heme. We show that the Has and Hxu systems are functional signal transduction pathways of the cell-surface signaling class and report the mechanism triggering the activation of these signaling systems. Both signaling cascades involve an outer membrane receptor (HasR and HxuA, respectively) that upon sensing heme in the extracellular medium produces the activation of an σECF factor in the cytosol. HxuA has a major role in signaling and a minor role in heme acquisition in conditions in which the HasR and PhuR receptors or other sources of iron are present. Remarkably, P. aeruginosa compensates the lack of the HasR receptor by increasing the production of HxuA, which underscores the importance of heme signaling for this pathogen. This article is protected by copyright. All rights reserved.
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影响因子：
11.3
作者：
Becker KW;Skaar EP
通讯作者：
Skaar EP
影响因子：
30.3
作者：
Cassat JE;Skaar EP
通讯作者：
Skaar EP
DOI：
10.1099/00221287-146-1-185
发表时间：
2000-01-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Ochsner, UA;Johnson, Z;Vasil, ML
通讯作者：
Vasil, ML
影响因子：
2.9
作者：
Smith AD;Modi AR;Sun S;Dawson JH;Wilks A
通讯作者：
Wilks A