Desmosomal proteins in cultured and intact human periodontal ligament fibroblasts.
Desmosomal proteins in cultured and intact human periodontal ligament fibroblasts.
复制标题
培养的和完整的人牙周膜成纤维细胞中的桥粒蛋白。
作者：
Y. Yamaoka;Y. Sawa;N. Ebata;S. Yoshida;T. Kawasaki
本研究检测了人牙周膜成纤维细胞(PDLF)中桥粒蛋白的种类。对年轻和老年患者的PDLF进行培养，并用抗桥粒蛋白、桥粒蛋白和桥粒粘连蛋白的抗体用ELISA法测定桥粒蛋白的含量。用相同的抗体对培养的人牙周膜细胞和组织切片进行免疫组化染色。ELISA法和免疫组织化学研究均显示PDLF中有桥粒蛋白的表达，提示PDLF中存在桥粒样连接。这些连接被认为是为了保护PDLF中的缝隙连接，防止细胞收缩引起的细胞转化，细胞收缩可能与牙齿萌出和牙周组织的修复有关，和/或强大的咬合力。在这项研究中，年轻和老年患者之间的桥粒和桥粒蛋白的表达有统计学意义的差异(P<；0.0001)。
This study examined the kinds of desmosomal proteins in the human periodontal ligament fibroblasts (PDLFs). The PDLFs obtained from young and older patients were cultured and the amounts of desmosomal proteins were measured by ELISA with antibodies to desmoplakins, desmogleins, and desmocollins. Cultured cells and tissue sections of the human periodontal ligament were immunostained with the same antibodies. Expression of desmosomal proteins in the PDLFs was clearly demonstrated both by ELISA and the immunohistochemical studies, suggesting the existence of desmosome-like junctions in the PDLFs. The junctions are considered to protect gap junctions in the PDLFs against cell transformation caused by cell contraction, which may relate to tooth eruption and repair of periodontal tissue, and/or strong occlusal forces. Statistically significant differences (P < 0.0001) in the expression of desmoplakins and desmogleins between younger and older patients were observed in this study.
DOI：
10.1073/pnas.89.2.544
发表时间：
1992-01-15
影响因子：
11.1
作者：
VIRATA, MLA;WAGNER, RM;GREEN, KJ
通讯作者：
GREEN, KJ