Mechanisms of Transport of p-Borono-Phenylalanine through the Cell Membrane In Vitro
Mechanisms of Transport of p-Borono-Phenylalanine through the Cell Membrane In Vitro
复制标题
对硼苯丙氨酸通过细胞膜的体外转运机制
作者：
A. Wittig;Wolfgang Sauerwein;J. Coderre
摘要Wittig，A.，Sauerwein，W.A.和Coderre，J.A.，p-B-苯丙氨酸通过细胞膜的转运机制。辐射。第153、173-180号决议(2000年)。研究了p-(二羟基硼)-苯丙氨酸(BPA)通过细胞膜的转运机制，特别是对作为BPA潜在载体的中性氨基酸转运系统进行了评价。将大鼠9L胶质肉瘤细胞和中国仓鼠V79细胞暴露于不含氨基酸的特定条件下的双酚A。测定不同条件下10B(双酚A)的摄取和外排的时间进程。用油过滤技术将细胞培养液中的细胞与胞外的硼分离后，用直流等离子体原子发射光谱仪(DCP-AES)分析细胞内的硼含量。研究了细胞类型、温度、氨基酸组成和浓度以及氨基酸转运系统的特定底物等因素对双酚A摄取和外排的影响。本研究结果支持双酚A由L系统转运的假说，并且L或A氨基酸转运系统在细胞内积累的氨基酸可进一步刺激双酚A的转运。
Abstract Wittig, A., Sauerwein, W.A. and Coderre, J.A. Mechanisms of Transport of p-Borono-Phenylalanine through the Cell Membrane. Radiat. Res. 153, 173–180 (2000). The mechanisms of transport of p-(dihydroxyboryl)-phenylalanine (BPA) through the cell membrane were investigated in vitro, evaluating especially the systems responsible for the transport of neutral amino acids as potential carriers for BPA. Rat 9L gliosarcoma cells and Chinese hamster V79 cells were exposed to BPA under controlled conditions and in a defined medium that was free of amino acids. The time course of 10B (delivered by BPA) uptake and efflux was measured under different conditions. To analyze the intracellular boron content, direct-current plasma atomic emission spectroscopy (DCP-AES) was used after separating the cells from extracellular boron in the cell medium using an oil filtration technique. The dependence of factors such as cell type, temperature, composition and concentration of amino acids and specific substrates for amino acid transport systems in the culture medium or in intracellular compartments on BPA uptake and efflux were studied. The results of this study support the hypothesis that BPA is transported by the L system and that transport can be further stimulated by amino acids preaccumulated in the cell by either the L or A amino acid transport system.
体外肿瘤细胞对基底膜的侵袭受到氨基酸的调节。
影响因子：
2.4
作者：
Singh,RK;Rinehart,CA;Kim,JP;Tolleson-Rinehart,S;Lawing,LF;Kaufman,DG;Siegal,GP
通讯作者：
Siegal,GP
硼苯丙氨酸选择性靶向 BALB/c 小鼠黑色素瘤，用于中子捕获治疗。
影响因子：
11.2
作者：
Coderre,JA;Glass,JD;Fairchild,RG;Roy,U;Cohen,S;Fand,I
通讯作者：
Fand,I
影响因子：
7.4
作者：
BARTH, RF;ADAMS, DM;ANISUZZAMAN, AKM
通讯作者：
ANISUZZAMAN, AKM