Envelope gene sequences encoding variable regions 3 and 4 are involved in macrophage tropism of feline immunodeficiency virus.
Envelope gene sequences encoding variable regions 3 and 4 are involved in macrophage tropism of feline immunodeficiency virus.
复制标题
编码可变区3和4的包膜基因序列涉及猫免疫缺陷病毒的巨噬细胞向性。
DOI：
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发表时间：
1999
影响因子：
3.8
通讯作者：
H. Egberink
中科院分区：
文献类型：
--
作者：
T. Vahlenkamp;A. De ronde;N. Schuurman;A. V. van Vliet;J. van Drunen;Marian C. Horzinek;H. Egberink
包膜对于猫免疫缺陷病毒（FIV）进入其宿主细胞至关重要，所述宿主细胞由免疫系统细胞（包括巨噬细胞）组成。为了表征参与巨噬细胞嗜性的包膜糖蛋白决定簇，构建了嵌合感染性分子克隆，其含有来自已在外周血单核细胞（PBMC）中繁殖的分离物的包膜基因序列。检查子代病毒在PBMC和骨髓源性巨噬细胞中的生长，并选择在巨噬细胞中具有不同复制动力学的病毒。包膜嵌合病毒显示，编码表面糖蛋白SU的可变区3和4的核苷酸序列参与FIV的巨噬细胞嗜性。为了评估这一发现的生物学重要性，检查了来自实验性FIV感染的健康猫的骨髓、脑、淋巴结和PBMC的病毒的包膜蛋白的表型。由于必须避免繁殖过程中的选择，因此直接扩增前病毒包膜基因序列并克隆到FIV株Petaluma的感染性分子克隆中。检查获得的病毒的复制特性。在测试的15个克隆中，13个克隆在PBMC和巨噬细胞中复制，两个（脑源性克隆）仅在PBMC中复制，并且没有在Crandell猫肾细胞或星形胶质细胞中复制。这些结果表明，PBMC和巨噬细胞的双重向性是体内存在的FIV变体的共同特征。
The envelope is of cardinal importance for the entry of feline immunodeficiency virus (FIV) into its host cells, which consist of cells of the immune system including macrophages. To characterize the envelope glycoprotein determinants involved in macrophage tropism, chimeric infectious molecular clones were constructed containing envelope gene sequences from isolates that had been propagated in peripheral blood mononuclear cells (PBMC). The progeny virus was examined for growth in PBMC and bone marrow-derived macrophages and viruses with different replication kinetics in macrophages were selected. Envelope-chimeric viruses revealed that nucleotide sequences encoding variable regions 3 and 4 of the surface glycoprotein, SU, are involved in macrophage tropism of FIV. To assess the biological importance of this finding, the phenotypes of envelope proteins of viruses derived from bone marrow, brain, lymph node and PBMC of an experimentally FIV-infected, healthy cat were examined. Since selection during propagation had to be avoided, provirus envelope gene sequences were amplified directly and cloned into an infectious molecular clone of FIV strain Petaluma. The viruses obtained were examined for their replication properties. Of 15 clones tested, 13 clones replicated both in PBMC and macrophages, two (brain-derived clones) replicated in PBMC only and none replicated in Crandell feline kidney cells or astrocytes. These results indicate that dual tropism for PBMC and macrophages is a common feature of FIV variants present in vivo.
影响因子：
1
作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen
通讯作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen
影响因子：
56.9
作者：
PEDERSEN, NC;HO, EW;YAMAMOTO, JK
通讯作者：
YAMAMOTO, JK