Trichodesmium-derived dissolved organic matter is a source of nitrogen capable of supporting the growth of toxic red tide Karenia brevis
Trichodesmium-derived dissolved organic matter is a source of nitrogen capable of supporting the growth of toxic red tide Karenia brevis
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毛藻衍生的溶解有机物是能够支持有毒赤潮短卡累尼藻生长的氮源
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
O. Schofield
中科院分区：
文献类型：
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作者：
R. E. Sipler;D. Bronk;S. Seitzinger;R. Lauck;L. McGuinness;G. Kirkpatrick;C. Heil;L. Kerkhof;O. Schofield
固氮菌Trichodesmium sp.产生的溶解有机氮（DON）具有作为赤潮甲藻Karenia brevis氮源的潜力。从束毛藻（Trichodesmium sp.）的实验室培养物中分离、浓缩溶解有机质（DOM），并将其作为营养源供K.从墨西哥湾采集的短膜细胞K.短束毛束菌的数量在加入细胞渗出物（TCE）后立即增加，使种群在最初的24小时内翻了一番。TCE DON和~89%的TCE溶解有机磷（DOP）被快速完全利用。此外，还采用了末端限制性片段长度多态性（TRFLP）来评估细菌群落对TCE添加的反应。细菌操作分类单位（OTU）的数量最初增加后，TCE DOM添加，但随着K。brevis达到了最大丰度。采用电喷雾电离质谱（ESI-MS）和傅里叶变换离子回旋共振质谱（FT-ICR MS）对DOM进行化学表征。大约25%的化合物在前24小时内消失，对应于K的最大增加。brevis丰度使用FT-ICR MS，391 DON和219 DOP潜在的生物利用度的化合物进行了表征。生物可利用的DON化合物高度减少，44%具有指示脂质或蛋白质样化合物的摩尔比。DON浓度和化合物组成的变化表明Trichodesmium sp.提供了足够的氮源来直接或间接支持K。短枝花
Dissolved organic nitrogen (DON) produced by the nitrogen-fixer Trichodesmium sp. has the potential to serve as a nitrogen source for the red tide dinoflagellate Karenia brevis. Dis- solved organic matter (DOM) from laboratory cultures of Trichodesmium sp. was isolated, concen- trated and then supplied as a nutrient source to K. brevis cells collected from the Gulf of Mexico. K. brevis abundance increased immediately after Trichodesmium sp. cellular exudate (TCE) addition, allowing the population to double within the first 24 h. There was rapid and complete utilization of the TCE DON as well as ~89% of the TCE dissolved organic phosphorus (DOP). Additionally, ter- minal restriction fragment length polymorphism (TRFLP) was used to assess the bacterial commu- nity response to the addition of TCE . The number of bacterial operational taxonomic units (OTUs) initially increased after the TCE DOM addition, but decreased as K. brevis reached its maximum abundance. Electrospray ionization mass spectrometry (ESI-MS) and Fourier transform ion cy- clotron resonance mass spectrometry (FT-ICR MS) were used to chemically characterize the DOM. Approximately 25% of compounds disappeared within the first 24 h, corresponding to the greatest increase in K. brevis abundance. Using FT-ICR MS, 391 DON and 219 DOP potentially bioavailable compounds were characterized. The bioavailable DON compounds were highly re- duced and 44% had molar ratios indicative of lipid or protein-like compounds. The changes in DON concentration and compound composition show that Tricho desmium sp. provides a sufficient source of nitrogen to directly or indirectly support K. brevis blooms.
影响因子：
6.6
作者：
Fleming LE;Kirkpatrick B;Backer LC;Walsh CJ;Nierenberg K;Clark J;Reich A;Hollenbeck J;Benson J;Cheng YS;Naar J;Pierce R;Bourdelais AJ;Abraham WM;Kirkpatrick G;Zaias J;Wanner A;Mendes E;Shalat S;Hoagland P;Stephan W;Bean J;Watkins S;Clarke T;Byrne M;Baden DG
通讯作者：
Baden DG
影响因子：
6.6
作者：
Brand, Larry E.;Campbell, Lisa;Bresnan, Eileen
通讯作者：
Bresnan, Eileen
墨西哥湾赤潮：地点、时间和原因？
影响因子：
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作者：
Walsh,JJ;Jolliff,JK;Darrow,BP;Lenes,JM;Milroy,SP;Remsen,A;Dieterle,DA;Carder,KL;Chen,FR;Vargo,GA;Weisberg,RH;Fanning,KA;Muller-Karger,FE;Shinn,E;Steidinger,KA;Heil,CA;Tomas,CR;Prospero,JS;Lee,TN;Kirkp
通讯作者：
Kirkp