Quantifying the mechanical and histological properties of thrombus analog made from human blood for the creation of synthetic thrombus for thrombectomy device testing.
Quantifying the mechanical and histological properties of thrombus analog made from human blood for the creation of synthetic thrombus for thrombectomy device testing.
复制标题
DOI：
10.1136/neurintsurg-2017-013675
复制
发表时间：
2018-12
影响因子：
4.8
通讯作者：
中科院分区：
文献类型：
--
作者：
未经治疗的缺血性卒中可导致严重的发病率和死亡，因此，有许多血管内血凝块清除（血栓切除术）器械获批用于人体。人类血栓类型是高度可变的，并且通常以定性术语分类-“软/红色”、“硬/白色"或”老化/钙化“。量化人体血栓性质可以加速血栓类似物的开发，用于血栓切除术结局的研究，这在治疗的患者中通常是不一致的。从离体血液样品（即，在样品小瓶中凝结的人血液）中产生“软”人血栓，并使用混合流变仪材料测试系统测试其机械性能。合成血栓材料也进行了机械测试，并与“软”人类血凝块进行了比较。机械测试量化了志愿者血栓样本的剪切模量和动态（弹性）模量。该数据用于配制由聚丙烯酰胺和藻酸盐的复合聚合物水凝胶（PAAM-Alg）制成的合成血凝块。PAAM-Alg互穿网络具有可调的弹性模量、剪切模量和形状记忆特性。由于其可调节的性质，PAAM-Alg可以被修饰以模拟各种血栓分类。未来的研究将包括获得患者血栓切除术样本并对其进行定量分类，以及改变PAAM-Alg以模拟用于体外血栓切除术研究的结果。
Untreated ischemic stroke can lead to severe morbidity and death, and as such, there are numerous endovascular blood-clot removal (thrombectomy) devices approved for human use. Human thrombi types are highly variable and are typically classified in qualitative terms – ‘soft/red,’ ‘hard/white,’ or ‘aged/calcified.’ Quantifying human thrombus properties can accelerate the development of thrombus analogs for the study of thrombectomy outcomes, which are often inconsistent among treated patients. ‘Soft’human thrombi were created from blood samples ex vivo (ie, human blood clotted in sample vials) and tested for mechanical properties using a hybrid rheometer material testing system. Synthetic thrombus materials were also mechanically tested and compared with the ‘soft’ human blood clots. Mechanical testing quantified the shear modulus and dynamic (elastic) modulus of volunteer human thrombus samples. This data was used to formulate a synthetic blood clot made from a composite polymer hydrogel of polyacrylamide and alginate (PAAM-Alg). The PAAM-Alg interpenetrating network of covalently and ionically cross-linked polymers had tunable elastic and shear moduli properties and shape memory characteristics. Due to its adjustable properties, PAAM-Alg can be modified to mimic various thrombi classifications. Future studies will include obtaining and quantitatively classifying patient thrombectomy samples and altering the PAAM-Alg to mimic the results for use with in vitro thrombectomy studies.
登录
查看更多内容
影响因子：
3.5
作者：
Kaesmacher, J.;Boeckh-Behrens, T.;Huber, T.
通讯作者：
Huber, T.
影响因子：
64.8
作者：
Sun, Jeong-Yun;Zhao, Xuanhe;Illeperuma, Widusha R. K.;Chaudhuri, Ovijit;Oh, Kyu Hwan;Mooney, David J.;Vlassak, Joost J.;Suo, Zhigang
通讯作者：
Suo, Zhigang
影响因子：
8.3
作者：
Marder, Victor J.;Chute, Dennis J.;Saver, Jeffrey L.
通讯作者：
Saver, Jeffrey L.
DOI：
10.1016/j.jstrokecerebrovasdis.2015.01.008
发表时间：
2015-06-01
影响因子：
2.5
作者：
Leker, Ronen R.;Pikis, Stelios;Cohen, Jose E.
通讯作者：
Cohen, Jose E.
DOI：
10.1002/jbm.10202
发表时间：
2002-09-15
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Becker, TA;Kipke, DR
通讯作者：
Kipke, DR