Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum field isolates from Myanmar.
Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum field isolates from Myanmar.
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DOI：
10.1186/1475-2875-9-131
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发表时间：
2010-05-17
期刊：
影响因子：
3
通讯作者：
Na BK
中科院分区：
文献类型：
--
作者：
Kang JM;Moon SU;Kim JY;Cho SH;Lin K;Sohn WM;Kim TS;Na BK
恶性疟原虫裂殖子表面蛋白-1（MSP-1）和MSP-2是疟疾疫苗的潜在候选抗原。然而，恶性疟原虫田间分离株中抗原的广泛遗传多态性是开发有效疫苗的主要障碍。本研究分析了缅甸恶性疟原虫野毒株MSP-1和MSP-2基因的遗传多态性。本研究共使用了63份恶性疟原虫感染的血液样本，这些样本采集自缅甸曼德勒省一家地区医院的患者。侧翼的高度多态性的字符，MSP-1和MSP-2的块3的区域，基因分型等位基因特异性巢式PCR分析寄生虫的人口多样性。MSP-1和MSP-2的多态性区域的序列分析也进行了鉴定等位基因多样性的寄生虫种群。MSP-1和MSP-2等位基因多态性在缅甸分离的恶性疟原虫中存在多样性，大多数感染被确定为混合感染。MSP-1基因2区序列分析表明，MSP-1基因有14个不同的等位基因，其中K1型5个，MAD 20型9个。对于MSP-2区组3，共鉴定出22个等位基因（7个为FC 27型，15个为3D 7型）。MSP-1和MSP-2在缅甸恶性疟原虫田间分离株中具有广泛的遗传多态性和不同的等位基因类型。还观察到混合感染率很高，感染的多重性也很高。
Merozoite surface protein-1 (MSP-1) and MSP-2 of Plasmodium falciparum are potential vaccine candidate antigens for malaria vaccine development. However, extensive genetic polymorphism of the antigens in field isolates of P. falciparum represents a major obstacle for the development of an effective vaccine. In this study, genetic polymorphism of MSP-1 and MSP-2 among P. falciparum field isolates from Myanmar was analysed. A total of 63 P. falciparum infected blood samples, which were collected from patients attending a regional hospital in Mandalay Division, Myanmar, were used in this study. The regions flanking the highly polymorphic characters, block 2 for MSP-1 and block 3 for MSP-2, were genotyped by allele-specific nested-PCR to analyse the population diversity of the parasite. Sequence analysis of the polymorphic regions of MSP-1 and MSP-2 was also conducted to identify allelic diversity in the parasite population. Diverse allelic polymorphism of MSP-1 and MSP-2 was identified in P. falciparum isolates from Myanmar and most of the infections were determined to be mixed infections. Sequence analysis of MSP-1 block 2 revealed that 14 different alleles for MSP-1 (5 for K1 type and 9 for MAD20 type) were identified. For MSP-2 block 3, a total of 22 alleles (7 for FC27 type and 15 for 3D7 type) were identified. Extensive genetic polymorphism with diverse allele types was identified in MSP-1 and MSP-2 in P. falciparum field isolates from Myanmar. A high level of mixed infections was also observed, as was a high degree of multiplicity of infection.
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影响因子：
3.6
作者：
Healer, J;Murphy, V;Batchelor, A
通讯作者：
Batchelor, A
DOI：
10.4269/ajtmh.2004.70.238
发表时间：
2004-03-01
影响因子：
3.3
作者：
Basco, LK;Tahar, R;Escalante, A
通讯作者：
Escalante, A
DOI：
10.4269/ajtmh.1995.52.81
发表时间：
1995-01-01
影响因子：
3.3
作者：
NTOUMI, F;CONTAMIN, H;MERCEREAUPUIJALON, O
通讯作者：
MERCEREAUPUIJALON, O
影响因子：
3.3
作者：
Peyerl-Hoffmann, G;Jelinek, T;von Sonnenburg, F
通讯作者：
von Sonnenburg, F
影响因子：
2.1
作者：
Ferreira, Marcelo U.;Hartl, Daniel L.
通讯作者：
Hartl, Daniel L.