Dynamic Changes of Mitochondrial Fusion and Fission in Brain Injury after Cardiac Arrest in Rats.
Dynamic Changes of Mitochondrial Fusion and Fission in Brain Injury after Cardiac Arrest in Rats.
复制标题
大鼠心脏骤停后脑损伤中线粒体融合与裂变的动态变化。
DOI：
10.1155/2017/1948070
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发表时间：
2017
影响因子：
--
通讯作者：
Huang Z
中科院分区：
文献类型：
--
作者：
Li Y;Tang Q;Wang P;Qin J;Wu H;Lin J;Huang Z
线粒体通过不断的融合和裂变过程动态地改变其形态以实现其功能。然而，关于心脏骤停对线粒体动力学的影响知之甚少。本研究旨在探讨心脏骤停大鼠脑缺血损伤后线粒体动力学的时间依赖性变化。复苏后，在心脏骤停后12 h和24 h，观察到明显的神经元损伤，三磷酸腺苷（ATP）水平降低，活性氧（ROS）生成过多，线粒体膜电位（MMP）下降，线粒体细胞色素c释放增加。此外，我们发现在心脏骤停后4小时观察到线粒体的伸长，而线粒体碎片化明显增加，同时在心脏骤停后12小时和24小时裂变蛋白Drp1和Fis1增加，融合蛋白Mfn1和Mfn2减少。综上所述，这些发现表明线粒体动力学的不平衡可能导致心脏骤停后的脑损伤。
Mitochondria change their morphology dynamically by continual fusion and fission processes to fulfill their function. However, little is known about the effect of cardiac arrest on mitochondrial dynamics. This study aimed to investigate time-dependent change of the mitochondrial dynamics after brain ischemic injury in rats of cardiac arrest. After resuscitation, obvious neuronal injury, reduced adenosine triphosphate (ATP) levels, excessive reactive oxygen species (ROS) generation, decreased mitochondrial membrane potential (MMP), and increased release of mitochondrial cytochrome c were observed at 12 h and 24 h after cardiac arrest. Moreover, we found that elongation of mitochondria was observed at 4 h after cardiac arrest, whereas fragmented mitochondria were significantly increased, along with concomitant increase in the fission proteins Drp1 and Fis1 and a reduction in the fusion proteins Mfn1 and Mfn2 at 12 h and 24 h after cardiac arrest. Taken together, these findings suggest that imbalance in mitochondrial dynamics probably contributes to brain injury after cardiac arrest.
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影响因子：
3
作者：
Parra, Valentina;Verdejo, Hugo;del Campo, Andrea;Pennanen, Christian;Kuzmicic, Jovan;Iglewski, Myriam;Hill, Joseph A.;Rothermel, Beverly A.;Lavandero, Sergio
通讯作者：
Lavandero, Sergio
影响因子：
37.8
作者：
Shenouda SM;Widlansky ME;Chen K;Xu G;Holbrook M;Tabit CE;Hamburg NM;Frame AA;Caiano TL;Kluge MA;Duess MA;Levit A;Kim B;Hartman ML;Joseph L;Shirihai OS;Vita JA
通讯作者：
Vita JA
影响因子：
9.8
作者：
Merrill RA;Dagda RK;Dickey AS;Cribbs JT;Green SH;Usachev YM;Strack S
通讯作者：
Strack S
影响因子：
3.5
作者：
Chen H;Chan DC
通讯作者：
Chan DC
影响因子：
9.9
作者：
Geocadin, R. G.;Buitrago, M. M.;Kaplan, P. W.
通讯作者：
Kaplan, P. W.