The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy.
The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy.
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DOI：
10.1186/1756-3305-6-298
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发表时间：
2013-10-14
影响因子：
3.2
通讯作者：
Maia MF
中科院分区：
文献类型：
--
作者：
Ntamatungiro AJ;Mayagaya VS;Rieben S;Moore SJ;Dowell FE;Maia MF
确定疟疾病媒的年龄对于评估减少野生蚊子种群生存的干预措施的影响和估计病媒能力的变化至关重要。近红外光谱法是一种简单、无损的方法，已被用于确定冈比亚按蚊的年龄和种类。通过分析吸收光谱的差异。光谱受蚊子生命期间发生的生物化学变化的影响，并且可能受衰老以及蚊子的生命史的影响，即，交配、吸血和产卵。为了更好地理解这些变化，我们评估了蚊子生理状态对近红外能量吸收光谱的影响。将蚊子保存在单独的杯中，以记录每种昆虫的生活史。蚊子的相同的实足年龄，但在不同的生理阶段，扫描和比较使用交叉验证。我们观察到在一些生理阶段内的轻微趋势，这表明较老的昆虫倾向于被预测为生理上更成熟。在校准中包括不同实足年龄和生理阶段的蚊子是有利的，因为它增加了模型的鲁棒性，从而得到更好的年龄预测。通过不同的生理状态的进展。阿拉伯藜对近红外光谱法预测实足年龄有影响。昆虫学家希望使用近红外技术来预测野外捕获的An。来自其研究区域的冈比亚s.l.应使用从其实地品系开发的校准，使用不同实足年龄和生理阶段的蚊子，以提高预测的稳健性和准确性。
Determining the age of malaria vectors is essential for evaluating the impact of interventions that reduce the survival of wild mosquito populations and for estimating changes in vectorial capacity. Near infra-red spectroscopy (NIRS) is a simple and non-destructive method that has been used to determine the age and species of Anopheles gambiae s.l. by analyzing differences in absorption spectra. The spectra are affected by biochemical changes that occur during the life of a mosquito and could be influenced by senescence and also the life history of the mosquito, i.e., mating, blood feeding and egg-laying events. To better understand these changes, we evaluated the influence of mosquito physiological status on NIR energy absorption spectra. Mosquitoes were kept in individual cups to permit record keeping of each individual insect’s life history. Mosquitoes of the same chronological age, but at different physiological stages, were scanned and compared using cross-validations. We observed a slight trend within some physiological stages that suggest older insects tend to be predicted as being physiologically more mature. It was advantageous to include mosquitoes of different chronological ages and physiological stages in calibrations, as it increases the robustness of the model resulting in better age predictions. Progression through different physiological statuses of An. arabiensis influences the chronological age prediction by the NIRS. Entomologists that wish to use NIR technology to predict the age of field-caught An. gambiae s.l from their study area should use a calibration developed from their field strain using mosquitoes of diverse chronological ages and physiological stages to increase the robustness and accuracy of the predictions.
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影响因子：
5.7
作者：
CLEMENTS, AN;PATERSON, GD
通讯作者：
PATERSON, GD
影响因子：
2.2
作者：
Aldrich, Benjamin T.;Maghirang, Elizabeth B.;Kambhampati, Srinivas
通讯作者：
Kambhampati, Srinivas
DOI：
10.4269/ajtmh.2009.09-0406
发表时间：
2009-12
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Doumbo OK;Thera MA;Koné AK;Raza A;Tempest LJ;Lyke KE;Plowe CV;Rowe JA
通讯作者：
Rowe JA
DOI：
10.4269/ajtmh.2005.73.553
发表时间：
2005-09-01
影响因子：
3.3
作者：
Gray, EM;Bradley, TJ
通讯作者：
Bradley, TJ
影响因子：
14.8
作者：
Cook, Peter E.;Hugo, Leon E.;O'Neill, Scott L.
通讯作者：
O'Neill, Scott L.