Extremely large and slowly processed precursors to the Euglena light-harvesting chlorophyll a/b binding proteins of photosystem II.
Extremely large and slowly processed precursors to the Euglena light-harvesting chlorophyll a/b binding proteins of photosystem II.
复制标题
光系统 II 的眼虫捕光叶绿素 a/b 结合蛋白的极大且缓慢加工的前体。
DOI：
10.1073/pnas.85.14.5117
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发表时间：
1988
影响因子：
11.1
通讯作者：
Schwartzbach,SD
中科院分区：
文献类型：
--
作者：
Rikin,A;Schwartzbach,SD
euglenallight -harvesting叶绿素a/b binding protein of photosystem II （LHCPII）抗体从总euglenaprotein中免疫沉淀207-、161-、122-和110-kDa蛋白，用[35S]硫酸盐脉冲标记10分钟。25.6 kda和27.2 kda的LHCPII在免疫沉淀物中几乎检测不到。在与未标记的硫酸盐进行40分钟的追逐过程中，高分子质量蛋白中的放射性量下降，而25.6和27.2 kda的LHCPII中的放射性量增加，其动力学与前体-产物关系一致。高分子质量蛋白的半衰期约为20 min。用全细胞或多聚(a)+RNA编程的核酸酶处理的兔网织红细胞无细胞翻译系统免疫沉淀的主要蛋白的分子质量与脉冲标记的体内翻译产物免疫沉淀的蛋白的分子质量相对应。6.6和8.3千碱基的rna是唯一与含有部分拟南芥hcpii编码序列的质粒pAB165的0.7千碱基ecori - bamhi片段杂交的全细胞和多(A)+ rna。这种大小的rna足以编码207kda的蛋白质。综上所述，这些发现表明，feugleni的lhcpii最初是作为分子质量为207、161、122和110 kDa的缓慢加工前体合成的。
Antibody to theEuglenalight-harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) immunoprecipitated 207-, 161-, 122-, and 110-kDa proteins from totalEuglenaproteins pulse-labeled for 10 min with [35S]sulfate. The 25.6- and 27.2-kDa LHCPII were barely detectable in the immunoprecipitate. During a 40-min chase with unlabeled sulfate, the amount of radioactivity in the high molecular mass proteins decreased, and the amount of radioactivity in the 25.6- and 27.2-kDa LHCPII increased with kinetics consistent with a precursor-product relationship. The half-life of the high molecular mass proteins was ≈20 min. The major proteins immunoprecipitated from a nuclease-treated rabbit reticulocyte cell-free translation system programmed withEuglenawhole cell or poly(A)+RNA had molecular masses corresponding to the molecular masses of the proteins immunoprecipitated from the pulse-labeledin vivotranslation products. RNAs of 6.6 and 8.3 kilobases were the onlyEuglenawhole cell and poly(A)+RNAs that hybridized to a 0.7-kilobaseEcoRI-BamHI fragment of plasmid pAB165, which contains a portion of the coding sequence forArabidopsisLHCPII. RNAs of this size are more than sufficient to code for proteins of 207 kDa. Taken together, these findings demonstrate that the LHCPIIs ofEuglenaare initially synthesized as slowly processed precursors with molecular masses of 207, 161, 122, and 110 kDa.
光合作用研究进展
DOI：
--
发表时间：
1984
期刊：
Advances in Agricultural Biotechnology
影响因子：
--
作者：
Kazuo Shibata
通讯作者：
Kazuo Shibata
影响因子：
4.3
作者：
H. Sambrook
通讯作者：
H. Sambrook