Overexpression of the transcription factor Rst2 in Schizosaccharomyces pombe indicates growth defect, mitotic defects, and microtubule disorder
Overexpression of the transcription factor Rst2 in Schizosaccharomyces pombe indicates growth defect, mitotic defects, and microtubule disorder
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粟酒裂殖酵母中转录因子 Rst2 的过度表达表明生长缺陷、有丝分裂缺陷和微管紊乱
DOI：
10.1080/09168451.2017.1415126
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yasuhiro Matsuo
中科院分区：
文献类型：
--
作者：
Kouhei Takenaka;Takuma Tanabe;M. Kawamukai;Yasuhiro Matsuo
摘要 在粟酒裂殖酵母中，转录因子 Rst2 在环磷酸腺苷 (cAMP) 依赖性蛋白激酶 (PKA) 途径下游调节减数分裂中的 ste11 和糖生成中的 fbp1。在这里，我们证明 Rst2 调节其他细胞事件。过表达的 Rst2 增加了椭圆形、弯曲、分支、分隔和多分隔细胞的频率。细胞表现出正常的核分裂，但在低频率下表现出异常的核组织。在卵圆细胞中，微管是弯曲的，但通过删除 mal3 可以挽救它们。由于 mal3 缺失不能挽救生长缺陷，因此我们认为它是独立调节的。功能性 Pka1 的丧失会加剧 Rst2 过表达时的生长缺陷，因为 Pka1 的下调作用消失了。 Rst2 的过度表达也会导致对 KCl 和 CaCl2 的敏感性。这些发现表明，除了减数分裂和糖生成之外，Rst2 还参与细胞生长、细胞形态、有丝分裂进展、微管结构、核结构和应激反应的调节等细胞事件。图解摘要 Rst2 的过度表达会加剧生长缺陷、对盐胁迫敏感、形态异常、细胞核异常、微管弯曲和孢子形成。
Abstract In Schizosaccharomyces pombe, the transcription factor Rst2 regulates ste11 in meiosis and fbp1 in glucogenesis downstream of the cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) pathway. Here, we demonstrate that Rst2 regulates additional cellular events. Overexpressed Rst2 elevated the frequency of oval, bent, branched, septated, and multi-septated cells. Cells showed normal nuclear divisions but exhibited abnormal nuclear organization at low frequency. In oval cells, microtubules were curved but they were rescued by the deletion of mal3. Since growth defect was not rescued by mal3 deletion, we argue that it is regulated independently. Loss of functional Pka1 exaggerated growth defect upon Rst2 overexpression because its downregulation by Pka1 was lost. Overexpression of Rst2 also caused sensitivity to KCl and CaCl2. These findings suggest that, in addition to meiosis and glucogenesis, Rst2 is involved in cellular events such as regulation of cell growth, cell morphology, mitosis progression, microtubules structure, nuclear structure, and stress response. Graphical Abstract Overexpression of Rst2 enhances growth defect, sensitive to salt stresses, abnormal morphology, abnormal nucleus, curved microtubule, and sporulation.
影响因子：
4
作者：
S. Byrne;C. S. Hoffman
通讯作者：
S. Byrne;C. S. Hoffman
粟酒裂殖酵母的转化：乙酸锂/二甲亚砜程序。
影响因子：
--
作者：
Murray JM
通讯作者：
Murray JM
DOI：
10.1016/j.bbapap.2007.10.002
发表时间：
2008-01-01
影响因子：
3.2
作者：
Taylor, Susan S.;Kim, Choel;Kannan, Natarajan
通讯作者：
Kannan, Natarajan