Second‐round interlaboratory comparison of hepatic ethoxyresorufin‐O‐deethylase activity in white sucker (Catostomus commersoni) exposed to bleached‐kraft pulp mill effluent
Second‐round interlaboratory comparison of hepatic ethoxyresorufin‐O‐deethylase activity in white sucker (Catostomus commersoni) exposed to bleached‐kraft pulp mill effluent
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暴露于漂白牛皮纸制浆厂废水的白吸盘（Catostomus commersoni）肝脏乙氧基试卤灵-O-脱乙基酶活性的第二轮实验室间比较
DOI：
10.1002/etc.5620140910
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发表时间：
1995
影响因子：
4.1
通讯作者：
J. Stegeman
中科院分区：
文献类型：
--
作者：
M. Heuvel;D. Dixon;K. Munkittrick;J. Stegeman
在 1992 年春季产卵迁徙期间，在漂白牛皮纸制浆厂废水 (BKME) 暴露地点和参考地点，从 6 只雄性和 6 只雌性白吸盘鱼 (Catostomus commersoni) 中采集肝脏。提出了一种用于 7-乙氧基试卤灵-O-脱乙基酶 (EROD) 分析的半标准化方法，该方法具有相似的物理和化学条件，但允许使用不同的仪器和技术。 14 个实验室对线粒体后上清液 (PMS) 进行了 EROD 活性分析，并在一个实验室内进行了重复 EROD 分析，以估计实验室内的变异性。所有实验室都发现，来自 BKME 暴露部位的男性的 EROD 活性水平显着升高（大约三倍）； 14 个实验室中只有 5 个发现暴露的女性体内的水平明显较高。由于异常值，来自三个实验室的数据被从分析中删除。其余 11 个实验室的平均变异系数 (C.V.) 为 34.7%，与 C.V. 没有显着差异。由一名技术人员 (28.4%) 进行六次分析确定。平均 C.V.一个实验室内 5 名分析师的数据显着低于前一个值 (16.4%)。微孔板和常规 EROD 测定数据的绝对值或变异性没有差异。 Bradford 和 Lowry 蛋白质数据的分析表明，两种检测方法的变异性较低（C.V.s 分别 = 16.8 和 17.4%）；这两种技术对蛋白质含量的估计非常相似。免疫化学分析表明微粒体P4501A浓度与EROD活性之间存在显着相关性。对市售试卤灵标准品的来源进行测试，平均摩尔消光系数为54.0±1.1 cm -1 mM -1 。
Livers were collected from six male and six female white sucker (Catostomus commersoni) at both a bleached-kraft pulp mill effluent (BKME)-exposed site and a reference site during the 1992 spring spawning migration. A semistandardized methodology for 7-ethoxyresorufin-O-deethylase (EROD) analysis was proposed that featured similar physical and chemical conditions yet allowed for different instrumentation and techniques. Postmitochondrial supernatants (PMSs) were analyzed for EROD activity by 14 laboratories, and repeat EROD analyses were performed within one laboratory to estimate within-laboratory variability. All laboratories found significantly higher levels of EROD activity (approximately threefold) in males from the BKME-exposed site ; only 5 of 14 laboratories found significantly higher levels in exposed females. Data from three labs were dropped from the analysis because of outlying values. The mean coefficient of variation (C.V.) for the remaining 11 laboratories was 34.7%, which was not significantly different from the C.V. determined for six analyses by one technician (28.4%). The mean C.V. for five analysts within one laboratory was significantly lower (16.4%) than either of the former values. There was no difference in absolute values or the variability of microplate and conventional EROD assay data. Analysis of Bradford and Lowry protein data showed that both assays have low variability (C.V.s = 16.8 and 17.4%, respectively) ; these two techniques produced very similar estimates of protein content. Immunochemical analysis demonstrated a significant correlation existed among microsomal P4501A concentration and EROD activity. Sources of commercially available resorufin standard were tested, and the mean molar extinction coefficient was 54.0 ± 1.1 cm -1 mM -1 .
邻位和非邻位取代的多氯联苯同系物对 scup（Stenotomus chrysops）肝单加氧酶系统的影响。
影响因子：
3.8
作者：
Gooch,JW;Elskus,AA;Kloepper-Sams,PJ;Hahn,ME;Stegeman,JJ
通讯作者：
Stegeman,JJ
冬鲽 (Pseudopleuronectes americanus) 和 scup (Stenotomus chrysops) 肝脏单加氧酶的性别差异以及雌二醇对 P450 形式的调节。
DOI：
10.1002/jez.1402590308
发表时间：
1991
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Gray,ES;Woodin,BR;Stegeman,JJ
通讯作者：
Stegeman,JJ
影响因子：
2.9
作者：
KLOTZ, AV;STEGEMAN, JJ;WALSH, C
通讯作者：
WALSH, C