The effect of cell penetrating peptides on transfection activity and cytotoxicity of polyallylamine.
The effect of cell penetrating peptides on transfection activity and cytotoxicity of polyallylamine.
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DOI：
10.15171/bi.2017.17
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Malaekeh-Nikouei B
中科院分区：
文献类型：
--
作者：
Sabouri-Rad S;Oskuee RK;Mahmoodi A;Gholami L;Malaekeh-Nikouei B
前言：阳离子聚合物有可能被修饰以获得理想的无病毒载体缺陷的基因载体。本研究的目的是通过引入细胞穿透肽(CPPs)来提高聚烯丙基胺(PAA)的转染率和降低细胞毒性。方法：分别用0.5%和1%的胺对PAA(15 KDa)进行TAT和CyLoP-1两种多肽修饰，制备多肽聚合物。考察了修饰聚合物的缓冲能力和DNA凝聚能力、纳米粒子的颗粒大小和Zeta电位、细胞存活率和载体的转染活性。结果：在载体与载体重量比(C/P)为0.5和1的情况下，未经修饰的聚合物比合成的载体具有更强的缩合DNA的能力。在C/P比为2的条件下，所有载体均能完全凝聚。多聚体的大小为195~240 nm。Zeta电位与PAA基本相同，变化范围为25~27 mV。与PAA相比，所有复合体都增加了缓冲容量。在C/P比为2的情况下，用1%的TAT或CyLoP-1多肽修饰的载体比未修饰的聚合物的转染率更高，细胞毒性低于PAA。在大多数情况下，CyLoP-1修饰样品的细胞毒性低于TAT修饰样品。结论：CPPS修饰PAA可提高载体的转染率。
Introduction: Cationic polymers have the potential to be modified to achieve an ideal gene vector lacking viral vector defects. The aim of the present study was to improve polyallylamine (PAA) transfection efficiency and to reduce cytotoxicity by incorporating of cell-penetrating peptides (CPPs). Methods: To prepare the peptide-based polyplexes, PAA (15 kDa) was modified with 2 peptides (TAT and CyLoP-1) by covering the 0.5% and 1% of amines. Buffer capacity and DNA condensation ability of modified polymer, particle size and zeta potential of nanoparticles, cell viability, and transfection activity of vectors were evaluated. Results: In low carrier to plasmid (C/P) weight ratios such as 0.5 and 1, the unmodified polymer was more capable to condense the DNA compared to the synthesized vectors. In C/P ratio of 2, the plasmid was fully condensed in all vectors. The size of polyplexes ranged from 195 to 240 nm. The zeta potential was almost as the same as PAA and varied from 25 to 27 mV. All polyplexes increased the buffer capacity compared to PAA. The transfection efficiency was improved compared to unmodified polymer especially in the vectors modified with 1% of TAT or CyLoP-1 peptides in C/P ratio of 2. The cytotoxicity of prepared vectors was less than PAA. In most ratios, the cytotoxicity of the CyLoP-1 modified samples was less than the TAT modified ones. Conclusion: Modification of PAA with CPPs improved the transfection activity of vector.
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DOI：
10.1016/s0167-4781(98)00274-7
发表时间：
1999-02-16
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Kwoh, DY;Coffin, CC;Carlo, DJ
通讯作者：
Carlo, DJ
影响因子：
6.2
作者：
Rungsardthong, U;Ehtezazi, T;Stolnik, S
通讯作者：
Stolnik, S
影响因子：
5.5
作者：
Munyendo WL;Lv H;Benza-Ingoula H;Baraza LD;Zhou J
通讯作者：
Zhou J
影响因子：
10.8
作者：
Chen, DJ;Majors, BS;Putnam, D
通讯作者：
Putnam, D
影响因子：
3.7
作者：
Fischer, D;Bieber, T;Kissel, T
通讯作者：
Kissel, T