Peptidoglycan and teichoic acid from Staphylococcus epidermidis stimulate human monocytes to release tumour necrosis factor-α, interleukin-1β and interleukin-6
Peptidoglycan and teichoic acid from Staphylococcus epidermidis stimulate human monocytes to release tumour necrosis factor-α, interleukin-1β and interleukin-6
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表皮葡萄球菌的肽聚糖和磷壁酸刺激人单核细胞释放肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
H. Dijk
中科院分区：
文献类型：
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作者：
E. Mattsson;L. Verhage;J. Rollof;A. Fleer;J. Verhoef;H. Dijk
细胞因子在感染性休克的病理生理学中起主要作用。在这项研究中，用从表皮葡萄球菌菌株中纯化的肽聚糖和磷壁酸刺激人类外周血单核细胞。加入多粘菌素B（PM-B）以避免可能的内毒素污染影响。采用酶联免疫吸附法测定上清液中肿瘤坏死因子-α（TNF）、白细胞介素-1 β（IL-1）和白细胞介素-6（IL-6）的含量。肽聚糖和磷壁酸以浓度依赖性方式诱导TNF、IL-1和IL-6。磷壁酸是比肽聚糖更弱的诱导剂，特别是对于IL-1。从E.大肠杆菌菌株用作对照，比肽聚糖和磷壁酸的效力高100-1000倍。
Cytokines play a major role in the pathophysiology of septic shock. In this study, human periferal blood monocytes were stimulated with peptidoglycan and teichoic acid, purified from a strain of Staphylococcus epidermidis. Polymyxin B (PM-B) was added to avoid the effects of possible contamination with endotoxin. Tumour necrosis factor-α (TNF), interleukin-1β (IL-1), and interleukin-6 (IL-6) in the supernates were measured by enzyme-linked immunosorbent assays. Peptidoglycan and teichoic acid induced TNF, IL-1, and IL-6 in a concentration-dependent manner. Teichoic acid was a weaker inducer than peptidoglycan, especially for IL-1. Lipopolysaccharide from an E. coli strain was used as a control, being 100–1000 times more potent than peptidoglycan and teichoic acid.
补充消耗淋球菌肽聚糖。
影响因子：
3.1
作者：
Petersen,BH;Rosenthal,RS
通讯作者：
Rosenthal,RS
影响因子：
15.9
作者：
WAKABAYASHI, G;GELFAND, JA;DINARELLO, CA
通讯作者：
DINARELLO, CA
影响因子：
20.3
作者：
R. Schindler;J. Mancilla;S. Endres;R. Ghorbani;S. Clark;C. Dinarello
通讯作者：
R. Schindler;J. Mancilla;S. Endres;R. Ghorbani;S. Clark;C. Dinarello