Genetic variation in Saccharomyces cerevisiae: circuit diversification in a signal transduction network.
Genetic variation in Saccharomyces cerevisiae: circuit diversification in a signal transduction network.
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DOI：
10.1534/genetics.112.145573
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发表时间：
2012-12
期刊：
影响因子：
3.3
通讯作者：
Fink GR
中科院分区：
文献类型：
--
作者：
Chin BL;Ryan O;Lewitter F;Boone C;Fink GR
通过使用两个相同的缺失文库确定两个密切相关的酵母菌株S288c和Sigma中相同突变的粘附表型来评估基因型和表型之间的联系。之前的研究，都在Sigma，已经表明，粘附表型是由促分裂原激活激酶（fMAPK）途径控制的，该途径激活了一组转录因子，这些转录因子是转录结构基因FLO11所需的。出乎意料的是，尽管fMAPK基因在其他途径中存在并具有活性，但在S288c中FLO11转录不需要fMAPK途径。使用转化和致敏的报告基因，可以分离RPI 1，其是允许绕过S288c中的fMAPK通路的修饰剂之一。RPI 1编码的转录因子在两种菌株之间具有等位基因差异：来自S288c的RPI 1等位基因而不是来自Sigma的RPI 1等位基因可以赋予FLO11的fMAPK途径独立转录。生化分析揭示了等位基因之间的磷酸化差异。在核苷酸水平上，这两个等位基因在ORF中串联重复的数目上不同。两种菌株之间的基因组比较显示，由于重复长度的变化，许多基因的大小不同。
The connection between genotype and phenotype was assessed by determining the adhesion phenotype for the same mutation in two closely related yeast strains, S288c and Sigma, using two identical deletion libraries. Previous studies, all in Sigma, had shown that the adhesion phenotype was controlled by the filamentation mitogen-activated kinase (fMAPK) pathway, which activates a set of transcription factors required for the transcription of the structural gene FLO11. Unexpectedly, the fMAPK pathway is not required for FLO11 transcription in S288c despite the fact that the fMAPK genes are present and active in other pathways. Using transformation and a sensitized reporter, it was possible to isolate RPI1, one of the modifiers that permits the bypass of the fMAPK pathway in S288c. RPI1 encodes a transcription factor with allelic differences between the two strains: The RPI1 allele from S288c but not the one from Sigma can confer fMAPK pathway-independent transcription of FLO11. Biochemical analysis reveals differences in phosphorylation between the alleles. At the nucleotide level the two alleles differ in the number of tandem repeats in the ORF. A comparison of genomes between the two strains shows that many genes differ in size due to variation in repeat length.
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影响因子：
4.5
作者：
Demogines, Ann;Smith, Erin;Kruglyak, Leonid;Alani, Eric
通讯作者：
Alani, Eric
DOI：
10.1073/pnas.88.21.9392
发表时间：
1991-11-01
影响因子：
11.1
作者：
ELION, EA;BRILL, JA;FINK, GR
通讯作者：
FINK, GR
影响因子：
64.8
作者：
Kellis, M;Patterson, N;Lander, ES
通讯作者：
Lander, ES
影响因子：
3.3
作者：
Connelly, Caitlin F.;Akey, Joshua M.
通讯作者：
Akey, Joshua M.
稀有变体会产生整个基因组的关联。
影响因子：
9.8
作者：
Dickson SP;Wang K;Krantz I;Hakonarson H;Goldstein DB
通讯作者：
Goldstein DB