Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America.
Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America.
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DOI：
10.1371/journal.pone.0272205
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Alejandra Picconi, Maria
中科院分区：
文献类型：
--
作者：
Mariel Correa, Rita;Baena, Armando;Valls, Joan;Celeste Colucci, Maria;Mendoza, Laura;Rol, Maryluz;Wiesner, Carolina;Ferrera, Annabelle;Dolores Fellner, Maria;Victor Gonzalez, Joaquin;Alejandro Basiletti, Jorge;Mongelos, Pamela;Rodriguez de la Pena, Mercedes;Saino, Agustina;Kasamatsu, Elena;Velarde, Carlos;Macavilca, Ninoska;Martinez, Sandra;Venegas, Gino;Calderon, Alejandro;Rodriguez, Guillermo;Barrios, Hernan;Herrero, Rolando;Almonte, Maribel;Alejandra Picconi, Maria
HPV 16和18相关宫颈癌（CC）的比例在全球范围内似乎相当稳定（≥70%），但其他HR-HPV的相对重要性因地理区域而略有不同。在这里，我们研究了HPV阳性的拉丁美洲（LA）妇女的HPV基因型分布的组织学分级，在一个子队列的ESTAMPA研究;我们还探讨了严重病变的年龄特异性HPV基因型的关联。通过两种PCR-反向印迹杂交策略对来自1，252名参与者（854名≤ CIN 1，121名CIN 2，194名CIN 3和83名CC）的宫颈样本进行基因分型：i）广谱通用引物5+/6+和ii）PGMY 9/11 PCR。HPV 16基因型在所有组织学分级中最常见，并随病变严重程度的增加而增加，从≤ CIN 1的14.5%、CIN 2的19.8%、CIN 3的51.5%到CC的65.1%（p < 0.001）。对于其余HR-HPV，与不太严重的类别相比，其在CC中的频率并未增加。CC中九价疫苗的HR型别居首位，主要为HPV 16和HPV 45。HR-HPV单一感染在≤ CIN 1和CIN 2中分别为57.1%和57.0%，在CIN 3和CC中分别为72.2%和91.6%（p<0.001）。在CC中未观察到年龄与HPV类型之间的相关性，尽管CIN 3病例中HPV 16感染的风险随年龄增加而增加。结果证实了HPV 16在整个临床谱中的相关性，其在CIN 3和癌症中的比例大幅上升。该信息将与评估HPV疫苗接种的影响相关，作为比较接种疫苗的女性参与洛杉矶地区筛查时HPV类型特异性分布的基因型变化的基线。同样，这些数据可以帮助选择最好的HPV检测系统，用于基于HPV的高效，负担得起和可持续的筛查计划。
The proportion of HPV16 and 18-associated cervical cancer (CC) appears rather constant worldwide (≥70%), but the relative importance of the other HR-HPV differs slightly by geographical region. Here, we studied the HPV genotype distribution of HPV positive Latin American (LA) women by histological grade, in a sub-cohort from the ESTAMPA study; we also explored the association of age-specific HPV genotypes in severe lesions. Cervical samples from 1,252 participants (854 ≤CIN1, 121 CIN2, 194 CIN3 and 83 CC) were genotyped by two PCRs-Reverse Blotting Hybridization strategies: i) Broad-Spectrum General Primers 5+/6+ and ii) PGMY9/11 PCRs. HPV16 was the most frequently found genotype in all histological grades, and increased with the severity of lesions from 14.5% in ≤ CIN1, 19.8% in CIN2, 51.5% in CIN3 to 65.1% in CC (p < 0.001). For the remaining HR-HPVs their frequency in CC did not increase when compared to less severe categories. The nonavalent vaccine HR-types ranked at the top in CC, the dominant ones being HPV16 and HPV45. HR-HPV single infection occurs, respectively, in 57.1% and 57.0% of ≤CIN1 and CIN2, increasing to 72.2% and 91.6% in CIN3 and CC (p<0.001). No association between age and HPV type was observed in CC, although the risk of HPV16 infection in CIN3 cases increased with age. Results confirm the relevance of HPV16 in the whole clinical spectrum, with a strong rise of its proportion in CIN3 and cancer. This information will be relevant in evaluating the impact of HPV vaccination, as a baseline against which to compare genotype changes in HPV type-specific distribution as vaccinated women participate in screening in LA region. Likewise, these data may help select the best HPV testing system for HPV-based efficient, affordable, and sustainable screening programmes.
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影响因子：
51.1
作者：
de Sanjose, Silvia;Quint, Wim G. V.;Xavier Bosch, F.
通讯作者：
Xavier Bosch, F.
影响因子：
6.4
作者：
Brotherton, Julia M. L.;Tabrizi, Sepehr N.;Garland, Suzanne M.
通讯作者：
Garland, Suzanne M.
影响因子：
2.9
作者：
Almonte, Maribel;Murillo, Raul;Herrero, Rolando
通讯作者：
Herrero, Rolando
影响因子：
3.4
作者：
Cuschieri, KS;Cubie, HA;McGoogan, E
通讯作者：
McGoogan, E
影响因子：
8.8
作者：
Bosch, F. X.;Castellsague, X.;de Sanjose, S.
通讯作者：
de Sanjose, S.