Pressure effects on the structure of lyotropic lipid mesophases and model biomembrane systems
Pressure effects on the structure of lyotropic lipid mesophases and model biomembrane systems
复制标题
压力对溶致脂质中间相结构和模型生物膜系统的影响
DOI：
10.1524/zkri.2000.215.8.454
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
C. Czeslik
中科院分区：
文献类型：
--
作者：
R. Winter;C. Czeslik
脂质系统为生物膜提供了有价值的模型系统，根据其分子结构和环境条件，脂质系统显示出多种多晶相。利用X射线和中子衍射研究了不同链构型和头基结构的脂类体系的温度和压力依赖性结构和相行为。除了层状相，也研究了非层状相。静水压力已被用作研究溶致脂质中间相稳定性和能量学的物理参数，而且还因为高压是某些天然膜环境的重要特征（例如，海洋生境）并且因为生物分子的高压相行为具有生物技术意义（例如，高压食品加工）。我们证明了温度和压力对结构和相行为的非一致性影响。通过结合时间分辨同步辐射X射线衍射的压力跃变弛豫技术，不同的脂质相变的动力学进行了研究。完成转变的时间常数取决于转变的方向、所涉及的结构的对称性和拓扑结构，以及压力跃变幅度。此外，将离子，类固醇和多肽的脂质系统的温度和压力依赖的相行为的双层的效果进行了讨论。
Lipid systems, which provide valuable model systems for biological membranes, display a variety of polymorphic phases, depending on their molecular structure and environmental conditions. By use of X-ray and neutron diffraction the temperature- and pressure-dependent structure and phase behavior of lipid systems, differing in chain configuration and headgroup structure, have been studied. Besides lamellar phases also nonlamellar phases have been investigated. Hydrostatic pressure has been used as a physical parameter for studying the stability and energetics of lyotropic lipid mesophases, but also because high pressure is an important feature of certain natural membrane environments (e.g., marine biotopes) and because the high pressure phase behavior of biomolecules is of biotechnological interest (e.g., high pressure food processing). We demonstrate that temperature and pressure have noncongruent effects on the structural and phase behavior. By using the pressure-jump relaxation technique in combination with time-resolved synchrotron X-ray diffraction, the kinetics of different lipid phase transformations was also investigated. The time constants for completion of the transitions depend on the direction of the transition, the symmetry and topology of the structures involved, and also on the pressure-jump amplitude. In addition, the effect of incorporating ions, steroids and polypeptides into bilayers on the temperature- and pressure-dependent phase behavior of the lipid systems is discussed.
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