Location of phosphotyrosine-containing proteins by immunocytochemistry in the rat forebrain corresponds to the distribution of the insulin receptor.
Location of phosphotyrosine-containing proteins by immunocytochemistry in the rat forebrain corresponds to the distribution of the insulin receptor.
复制标题
通过免疫细胞化学测定大鼠前脑中含有磷酸酪氨酸的蛋白质的位置对应于胰岛素受体的分布。
DOI：
10.1073/pnas.87.12.4453
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发表时间：
1990
影响因子：
11.1
通讯作者：
Livingston,JN
中科院分区：
文献类型：
--
作者：
Moss,AM;Unger,JW;Moxley,RT;Livingston,JN
通过某些生长因子受体和原癌基因产物的细胞调节涉及酪氨酸激酶活性以及由此产生的蛋白质底物的酪氨酸磷酸化。在本报告中，我们描述了分布的磷酸酪氨酸含材料检测免疫细胞化学（ICC）在大鼠前脑。通过磷酸酪氨酸和磷酸对硝基苯酯而不是磷酸丝氨酸、磷酸苏氨酸或L-酪氨酸抑制免疫染色反应的能力证明了ICC技术中使用的针对磷酸酪氨酸的亲和纯化抗体的特异性。与ICC，相对较高的磷酸酪氨酸阳性物质，观察到在特定的结构，包括视上核，室旁核，弓状核，正中隆起，内侧缰，穹窿下器官和梨状皮质的神经元。在大脑皮层II-IV层和海马锥体细胞层中存在中度至高度含量。在其他几个地点也检测到少量至中等数量的农药。神经胶质细胞成分显示极轻微的染色。大鼠前脑的其他区域未能与这种抗体反应。重要的是，磷酸酪氨酸阳性区域的分布在很大程度上与脑胰岛素受体的分布一致，而脑胰岛素受体本身具有酪氨酸激酶活性。这些发现表明胰岛素受体和这些神经元的磷酸酪氨酸含量增加之间的关系，并支持脑胰岛素受体在体内是活跃的这一概念。
Cellular regulation by certain growth factor receptors and protooncogene products involves tyrosine kinase activity with the resultant tyrosine phosphorylation of protein substrates. In the present report we describe the distribution of phosphotyrosine-containing material detected by immunocytochemistry (ICC) in the rat forebrain. Specificity of the affinity-purified antibody against phosphotyrosine used in the ICC technique was demonstrated by the ability of phosphotyrosine and p-nitrophenyl phosphate but not phosphoserine, phosphothreonine, or L-tyrosine to inhibit the immunostaining reaction. With ICC, relatively high amounts of phosphotyrosine-positive material were observed in neurons in specific structures that included the supraoptic, paraventricular, and arcuate nuclei; the median eminence; medial habenula; subfornical organ; and piriform cortex. Moderate to high amounts were present in the cerebral cortical layers II-IV and in the pyramidal cell layer of the hippocampus. Small to moderate amounts were detected in a few other locations. Glial elements showed minimal staining. Other areas of the rat forebrain failed to react with this antibody. Importantly, the distribution of the areas positive for phosphotyrosine agreed to a remarkable extent with the distribution of the brain insulin receptor, which itself has tyrosine kinase activity. These findings suggest a relationship between the insulin receptor and the increased phosphotyrosine content of these neurons and support the concept that the brain insulin receptor is active in vivo.
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影响因子：
8.8
作者：
Morgan, J E;Honess, D J;Bleehen, N M
通讯作者：
Bleehen, N M
葡萄糖、半乳糖或甘露糖可防止中国仓鼠卵巢细胞热细胞死亡。
影响因子：
11.2
作者：
Henle,KJ;Monson,TP;Moss,AJ;Nagle,WA
通讯作者：
Nagle,WA
高温与癌症
DOI：
--
发表时间：
1982
期刊：
Springer US
影响因子：
--
作者：
G. Hahn
通讯作者：
G. Hahn
用各种铂配位化合物处理的中国仓鼠卵巢细胞中 DNA 交联的诱导和修复与铂与 DNA 的结合、细胞毒性、致突变性和抗肿瘤活性有关。
影响因子：
11.2
作者：
A. C. Plooy;M. Dijk;P. Lohman
通讯作者：
P. Lohman
在体外防止多元醇热诱导的细胞杀伤。
影响因子：
11.2
作者：
Henle,KJ;Peck,JW;Higashikubo,R
通讯作者：
Higashikubo,R