Cellulose mediates attachment of Salmonella enterica Serovar Typhimurium to tomatoes.

Cellulose mediates attachment of Salmonella enterica Serovar Typhimurium to tomatoes.
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纤维素介导肠沙门氏菌鼠伤寒血清型对番茄的附着。

DOI:
10.1111/j.1758-2229.2011.00263.x
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发表时间:
2011
影响因子:
3.3
通讯作者:
Shaw RK
Shaw RK
中科院分区:
生物学3区
文献类型:
--
作者:
Shaw RK

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新鲜水果和蔬菜是健康均衡饮食的重要组成部分。然而,它们越来越被认为是传播传统上被归类为人畜共患病的人类病原体的重要媒介。我们对人类病原体在水果和蔬菜上或水果和蔬菜中定植和存活的机制的认识和理解存在重大差距。在这项研究中,我们研究了肠道沙门氏菌与番茄果实(Solanum lycopersicum)的结合,番茄果实正成为人类感染的主要来源。我们报告了沙门氏菌的鼠伤寒和森夫滕贝格血清型以聚集的方式粘附在未成熟的西红柿表面,而汤普森血清型则以扩散的方式粘附。我们发现,虽然鞭毛没有结合的作用,bcsCS。缺乏纤维素生产的Typhimurim突变体表现出对番茄的粘附水平显著降低。突变的反式互补使粘附恢复到野生型水平。
Fresh fruit and vegetables are important components of a healthy and balanced diet. However, they are increasingly being recognized as important vehicles for transmission of human pathogens that were traditionally classified as zoonotic. There is a significant gap in our knowledge and understanding of the mechanisms by which human pathogens colonize and survive on or in fruits and vegetables. In this study we investigated the binding ofSalmonella entericato tomato fruits (Solanum lycopersicum), which is becoming a major source of human infection. We report thatSalmonella entericaserovars Typhimurium and Senftenberg bound to the surface of unripe tomatoes in an aggregative pattern, while serovar Thompson adhered diffusely. We found that while flagella did not have a role in binding,bcsCS. Typhimurim mutants, deficient in cellulose production, exhibited significantly reduced level of attachment to tomatoes.Transcomplementation of the mutation restored adhesion to the wild‐type level.
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