Whole-genome sequencing, annotation, and biological characterization of a novel Siphoviridae phage against multi-drug resistant Propionibacterium acne.
Whole-genome sequencing, annotation, and biological characterization of a novel Siphoviridae phage against multi-drug resistant Propionibacterium acne.
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DOI：
10.3389/fmicb.2022.1065386
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发表时间：
2022
影响因子：
5.2
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Liao D;Zhang J;Liu R;Chen K;Liu Y;Shao Y;Shi X;Zhang Y;Yang Z
痤疮丙酸杆菌（Propionibacterium acne，P. acne）是一种对抗生素具有耐药性的细菌，它能引起严重的寻常痤疮，严重威胁公众健康和心理健康。从新桥医院污水处理中心分离到一株新的感染痤疮丙酸杆菌的溶菌噬菌体φ PaP 11 - 13。在双层琼脂平板上可形成直径为1.0~5.0mm的透明菌斑，对寄主具有较强的裂解能力。透射电子显微镜（TEM）观察表明，φ PaP11 - 13属于Siphoviridae科病毒（头径60 ± 4.5 nm，尾长170 ± 6.4 nm，尾宽14 ± 2.4 nm）。一步生长曲线显示潜伏期为5 h，爆发量为26 PFU/cell。此外，它在广泛的pH值和温度范围内表现出耐受性，但通过紫外线（UV）照射1小时后完全失活。全基因组测序结果显示，φ PaP11 - 13的dsDNA为线性结构，长度为29,648 bp。G/C含量为54.08%。经在线注释，共获得45个开放阅读框架（ORF）。本研究报道了一种新的痤疮丙酸杆菌噬菌体φ PaP11 - 13，它具有强大的裂解能力，无毒力因子，稳定性好。φ PaP11 - 13的表征和基因组分析将发展我们对噬菌体生物学和多样性的理解，并为控制抗生素耐药性痤疮丙酸杆菌诱导的重度寻常痤疮提供潜在的武器库。
Antibiotics-resistant Propionibacterium acne (P. acne) causes severe acne vulgaris, serious public health, and psychological threat. A new lytic bacteriophage (phage), φPaP11-13, infecting P. acne, was isolated from the sewage management center of Xinqiao Hospital. It can form transparent plaque with diameters of 1.0 ~ 5.0 mm on the double-layer agar plate, indicating a robust lytic ability against its host. Transmission electron microscopy (TEM) showed that φPaP11-13 belonged to the Siphoviridae family (head diameter 60 ± 4.5 nm, tail length 170 ± 6.4 nm, tail width 14 ± 2.4 nm). The one-step growth curve showed the incubation period was 5 h, and the burst size was 26 PFU (plaque-forming unit)/cell. Moreover, it exhibited tolerance over a broad range of pH and temperature ranges but was utterly inactivated by ultraviolet (UV) irradiation for 1 h. The whole-genome sequencing results revealed φPaP11-13 had a linear dsDNA with 29,648 bp length. The G/C content was 54.08%. Non-coding RNA genes and virulence factors were not found. Forty five open reading frames (ORFs) were identified after online annotation. This study reports a novel P. acne phage φPaP11-13, which has a robust lytic ability, no virulence factors, and good stability. The characterization and genomic analysis of φPaP11-13 will develop our understanding of phage biology and diversity and provide a potential arsenal for controlling antibiotics-resistant P. acne-induced severe acne vulgaris.
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影响因子：
4.6
作者：
Garneau JR;Depardieu F;Fortier LC;Bikard D;Monot M
通讯作者：
Monot M
DOI：
10.3390/molecules21081063
发表时间：
2016-08-13
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Fox L;Csongradi C;Aucamp M;du Plessis J;Gerber M
通讯作者：
Gerber M
影响因子：
3.6
作者：
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通讯作者：
Seite, Sophie
影响因子：
3.6
作者：
Fernandes, Sofia;Sao-Jose, Carlos
通讯作者：
Sao-Jose, Carlos
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro