Organization and variation analysis of 5S rDNA in different ploidy-level hybrids of red crucian carp × topmouth culter.
Organization and variation analysis of 5S rDNA in different ploidy-level hybrids of red crucian carp × topmouth culter.
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红鲫鱼和顶嘴鲌不同倍性杂交体中 5S rDNA 的组织和变异分析。
DOI：
10.1371/journal.pone.0038976
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
He W;Qin Q;Liu S;Li T;Wang J;Xiao J;Xie L;Zhang C;Liu Y
通过远缘杂交，对红鲫（Carassius auratus red var.，本研究成功地培育出了鲤亚科（Cyprininae，2n = 100）×翘嘴卡尔特（Erythro卡尔特ilishaeformis Bleeker，TC）的杂交后代。 二倍体杂种有74条染色体，分别来自RCC和TC;三倍体杂种有124条染色体，分别来自RCC和TC;四倍体杂种有148条染色体，分别来自RCC和TC。对3个不同倍性水平的杂种及其亲本的5SrDNA进行测序分析。RCC中存在3种单体5SrDNA类型（I类：203 bp; II类：340 bp; III类：477 bp），TC中存在2种单体5SrDNA类型（IV类：188 bp; V类：286 bp）。在杂种后代中，二倍体杂种从其母本（RCC）继承了3个5SrDNA类别，而从其父本（TC）只继承了IV类。三倍体杂种从母本（RCC）遗传II类和III类，从父本（TC）遗传IV类。四倍体杂种从其母本（RCC）获得了II类和III类，并产生了一个新的5S rDNA序列（命名为I-N类）。在杂种后代中未发现V类的父本特异5SrDNA序列。5SrDNA序列分析揭示了杂交和多倍化对鱼类5SrDNA组织和变异的影响。这是首次报道不同染色体数目的亲本杂交产生的二倍体、三倍体和四倍体杂种在脊椎动物中共存，这些新的杂种是研究第一代种间杂种基因组变异的新标本，对进化和鱼类遗传学具有重要意义。
Through distant crossing, diploid, triploid and tetraploid hybrids of red crucian carp (Carassius auratus red var., RCC♀, Cyprininae, 2n = 100) × topmouth culter (Erythroculter ilishaeformis Bleeker, TC♂, Cultrinae, 2n = 48) were successfully produced. Diploid hybrids possessed 74 chromosomes with one set from RCC and one set from TC; triploid hybrids harbored 124 chromosomes with two sets from RCC and one set from TC; tetraploid hybrids had 148 chromosomes with two sets from RCC and two sets from TC. The 5S rDNA of the three different ploidy-level hybrids and their parents were sequenced and analyzed. There were three monomeric 5S rDNA classes (designated class I: 203 bp; class II: 340 bp; and class III: 477 bp) in RCC and two monomeric 5S rDNA classes (designated class IV: 188 bp, and class V: 286 bp) in TC. In the hybrid offspring, diploid hybrids inherited three 5S rDNA classes from their female parent (RCC) and only class IV from their male parent (TC). Triploid hybrids inherited class II and class III from their female parent (RCC) and class IV from their male parent (TC). Tetraploid hybrids gained class II and class III from their female parent (RCC), and generated a new 5S rDNA sequence (designated class I–N). The specific paternal 5S rDNA sequence of class V was not found in the hybrid offspring. Sequence analysis of 5S rDNA revealed the influence of hybridization and polyploidization on the organization and variation of 5S rDNA in fish. This is the first report on the coexistence in vertebrates of viable diploid, triploid and tetraploid hybrids produced by crossing parents with different chromosome numbers, and these new hybrids are novel specimens for studying the genomic variation in the first generation of interspecific hybrids, which has significance for evolution and fish genetics.
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影响因子：
3.1
作者：
Campo, Daniel;Garcia-Vazquez, Eva
通讯作者：
Garcia-Vazquez, Eva
影响因子：
3.1
作者：
Gornung, Ekaterina;Colangelo, Paolo;Annesi, Flavia
通讯作者：
Annesi, Flavia
影响因子：
2.7
作者：
KOMIYA, H;HASEGAWA, M;TAKEMURA, S
通讯作者：
TAKEMURA, S
影响因子：
3.3
作者：
Liu, Shaojun;Qin, Qinbo;Liu, Yun
通讯作者：
Liu, Yun
影响因子：
6.2
作者：
Leggatt, RA;Iwama, GK
通讯作者：
Iwama, GK