Bottleneck in secretion of α-amylase in Bacillus subtilis.
Bottleneck in secretion of α-amylase in Bacillus subtilis.
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枯草芽孢杆菌α-淀粉酶分泌的瓶颈
DOI：
10.1186/s12934-017-0738-1
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发表时间：
2017-07-19
影响因子：
6.4
通讯作者：
Wu G
中科院分区：
文献类型：
--
作者：
Yan S;Wu G
淀粉酶在生物技术产业中发挥着重要作用，革兰氏阳性杆菌枯草芽孢杆菌是产生异质α-淀粉酶的主要宿主。然而，分泌压力限制了枯草芽孢杆菌α-淀粉酶的高产率，尽管人们已经为解决这一分泌瓶颈做出了巨大的努力。在本次以问题为导向的综述中，通过文献综述，力求回答以下看似简单但长期存在的问题：(1)α-淀粉酶是否需要特异性的、专用的伴侣？(2) CsaA识别哪些信号序列？(3) CsaA的运作是否需要ATP ？(4)未展开的α-淀粉酶是否比折叠的α-淀粉酶更难溶解？(5) α-淀粉酶在通过Sec分泌系统转运前是否聚集？(6) α-淀粉酶是否足够稳定以防止自身发生错误折叠？(7) α-淀粉酶是否需要更多的二硫键来稳定？(8) PrsA通过哪个分泌系统？(9) PrsA是否依赖于atp ？（10） α-淀粉酶折叠后，PrsA是否重复使用？（11） PrsA的命运如何？（12）触发因子（TF）是否依赖于atp ？文献综述表明，这些问题大部分仍有待解答，而且有必要计算ATP预算，以便更好地了解枯草芽孢杆菌如何利用其能量进行生产和分泌。
Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In this question-oriented review, every effort is made to answer the following questions, which look simple but are long-standing, through reviewing of literature: (1) Does α-amylase need a specific and dedicated chaperone? (2) What signal sequence does CsaA recognize? (3) Does CsaA require ATP for its operation? (4) Does an unfolded α-amylase is less soluble than a folded one? (5) Does α-amylase aggregate before transporting through Sec secretion system? (6) Is α-amylase sufficient stable to prevent itself from misfolding? (7) Does α-amylase need more disulfide bonds to be stabilized? (8) Which secretion system does PrsA pass through? (9) Is PrsA ATP-dependent? (10) Is PrsA reused after folding of α-amylase? (11) What is the fate of PrsA? (12) Is trigger factor (TF) ATP-dependent? The literature review suggests that not only the most of those questions are still open to answers but also it is necessary to calculate ATP budget in order to better understand how B. subtilis uses its energy for production and secretion.
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DOI：
10.1590/s1517-83822014000100014
发表时间：
2014
期刊：
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子：
--
作者：
Dey TB;Banerjee R
通讯作者：
Banerjee R
用于改善枯草芽孢杆菌异源蛋白分泌的组合 Sec 途径分析：通过系统基因过表达识别瓶颈。
影响因子：
6.4
作者：
Chen J;Fu G;Gai Y;Zheng P;Zhang D;Wen J
通讯作者：
Wen J
影响因子：
11.4
作者：
Dartigalongue, C;Raina, S
通讯作者：
Raina, S
影响因子：
4.8
作者：
Bolhuis, A;Venema, G;van Dijl, JM
通讯作者：
van Dijl, JM
影响因子：
2.4
作者：
Date, M;Itaya, H;Kikuchi, Y
通讯作者：
Kikuchi, Y