Nutritional upgrading for omnivorous carpenter ants by the endosymbiont Blochmannia.
Nutritional upgrading for omnivorous carpenter ants by the endosymbiont Blochmannia.
复制标题
内共体Blochmannia的杂食木蚂蚁的营养升级。
DOI：
10.1186/1741-7007-5-48
复制
发表时间：
2007-10-30
期刊：
影响因子：
5.4
通讯作者：
Gross, Roy
中科院分区：
文献类型：
--
作者：
Feldhaar, Heike;Straka, Josef;Krischke, Markus;Berthold, Kristina;Stoll, Sascha;Mueller, Martin J.;Gross, Roy
木蚁（弓背蚁属）被认为是杂食动物。尽管如此，Flochmannia floridanus（弓背蚁的专性细胞内内共生体）的基因组序列表明该细菌在宿主资源的营养升级中具有功能。因此，内共生体的基因组大幅减少，保留了功能性脲酶所有亚基的基因，以及宿主必需氨基酸（除了一种（精氨酸）之外的所有氨基酸）的生物合成途径的基因。 Blochmannia 的营养升级在 90 天的饲养实验中进行了测试，在工群中进行育雏试验，采用含有或不含必需氨基酸的化学限定日粮，并接受或不接受抗生素治疗。对照组喂蟑螂、蜂蜜水和巴特卡琼脂。向工蚁群提供从皇后右方母群收集的幼虫（每个蚁群 45 个卵和 45 个一龄幼虫）。在含有和不含必需氨基酸饮食的共生工蚁组之间，产卵量没有显着差异。然而，非共生工蚁群体在缺乏必需氨基酸的饮食中饲养的幼崽数量显着减少。当非共生工蚁的饮食中提供必需氨基酸时，这些工蚁减少的产仔产量得到了补偿。 90 天实验期后，通过 qRT-PCR 和 FISH 监测抗生素治疗导致的内共生菌减少。通过使用 15N 标记尿素的饲喂实验证实了脲酶功能。对工人体内游离氨基酸 15N 富集的 GC-MS 分析显示，非必需氨基酸丙氨酸、甘氨酸、天冬氨酸和谷氨酸以及必需氨基酸蛋氨酸和苯丙氨酸有显着标记。我们的结果表明，内共生 Blochmannia 可以对 C. floridanus 宿主的饮食进行营养升级，以提供必需氨基酸，并且它还可能通过其功能性脲酶在氮循环中发挥作用。布洛曼尼亚可能通过增强弓背蚁与其他缺乏这种内共生体的蚂蚁物种的竞争能力，通过营养升级赋予显着的适应性优势。内共生体的驯化可能促进了弓背蚁属的进化成功。
Carpenter ants (genus Camponotus) are considered to be omnivores. Nonetheless, the genome sequence of Blochmannia floridanus, the obligate intracellular endosymbiont of Camponotus floridanus, suggests a function in nutritional upgrading of host resources by the bacterium. Thus, the strongly reduced genome of the endosymbiont retains genes for all subunits of a functional urease, as well as those for biosynthetic pathways for all but one (arginine) of the amino acids essential to the host. Nutritional upgrading by Blochmannia was tested in 90-day feeding experiments with brood-raising in worker-groups on chemically defined diets with and without essential amino acids and treated or not with antibiotics. Control groups were fed with cockroaches, honey water and Bhatkar agar. Worker-groups were provided with brood collected from the queenright mother-colonies (45 eggs and 45 first instar larvae each). Brood production did not differ significantly between groups of symbiotic workers on diets with and without essential amino acids. However, aposymbiotic worker groups raised significantly less brood on a diet lacking essential amino acids. Reduced brood production by aposymbiotic workers was compensated when those groups were provided with essential amino acids in their diet. Decrease of endosymbionts due to treatment with antibiotic was monitored by qRT-PCR and FISH after the 90-day experimental period. Urease function was confirmed by feeding experiments using 15N-labelled urea. GC-MS analysis of 15N-enrichment of free amino acids in workers revealed significant labelling of the non-essential amino acids alanine, glycine, aspartic acid, and glutamic acid, as well as of the essential amino acids methionine and phenylalanine. Our results show that endosymbiotic Blochmannia nutritionally upgrade the diet of C. floridanus hosts to provide essential amino acids, and that it may also play a role in nitrogen recycling via its functional urease. Blochmannia may confer a significant fitness advantage via nutritional upgrading by enhancing competitive ability of Camponotus with other ant species lacking such an endosymbiont. Domestication of the endosymbiont may have facilitated the evolutionary success of the genus Camponotus.
登录
查看更多内容
影响因子：
30.8
作者：
Akman, L;Yamashita, A;Aksoy, S
通讯作者：
Aksoy, S
影响因子：
56.9
作者：
Davidson, DW;Cook, SC;Chua, TH
通讯作者：
Chua, TH
影响因子：
2.2
作者：
NONACS, P
通讯作者：
NONACS, P
影响因子：
1.4
作者：
BHATKAR A;WHITCOMB W H
通讯作者：
WHITCOMB W H
影响因子：
7
作者：
Degnan, PH;Lazarus, AB;Wernegreen, JJ
通讯作者：
Wernegreen, JJ