Engineering genome-reduced Bacillus subtilis for acetoin production from xylose
Engineering genome-reduced Bacillus subtilis for acetoin production from xylose
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基因组减少的枯草芽孢杆菌用于从木糖生产乙偶姻
DOI：
10.1007/s10529-017-2481-4
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发表时间：
2017-12
影响因子：
2.7
通讯作者：
Chen Tao
中科院分区：
文献类型：
--
作者：
Yan Panpan;Wu Yuanqing;Yang Li;Wang Zhiwen;Chen Tao
研究一株基因组精简的枯草芽孢杆菌菌株作为从木糖生产乙偶姻的底盘细胞的能力。为了赋予基因组精简的枯草芽孢杆菌菌株BSK814利用木糖的能力，我们插入了一个天然的木糖操纵子。
Objectives:To investigate the capacity of a genome-reduced Bacillus subtilis strain as chassis cell for acetoin production from xylose.Results:To endow the genome-reduced Bacillus subtilis strain BSK814 with the ability to utilize xylose, we inserted a native xyl operon into its genome and deleted the araR gene. The resulting strain BSK814A2 produced 2.94 g acetoin/l from 10 g xylose/l, which was 39% higher than control strain BSK19A2. The deletion of the bdhA and acoA genes further improved xylose utilization efficiency and increased acetoin production to 3.71 g/l in BSK814A4. Finally, BSK814A4 produced up to 23.3 g acetoin/l from 50 g xylose/l, with a yield of 0.46 g/g xylose. Both the titer and yield were 39% higher than those of control strain BSK19A4.Conclusions:As a chassis cell, genome-reduced B. subtilis showed significantly improved capacity for the production of the overflow product acetoin from xylose compared with wild-type strain.
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影响因子：
6.4
作者：
Lee JH;Sung BH;Kim MS;Blattner FR;Yoon BH;Kim JH;Kim SC
通讯作者：
Kim SC
基因组减少的枯草芽孢杆菌菌株的表征及其在鸟苷和胸苷生产中的应用
影响因子：
6.4
作者：
Li Y;Zhu X;Zhang X;Fu J;Wang Z;Chen T;Zhao X
通讯作者：
Zhao X
影响因子：
6.3
作者：
Förster AH;Beblawy S;Golitsch F;Gescher J
通讯作者：
Gescher J
通过代谢工程阴沟肠杆菌从木质纤维素资源中生物技术生产乙偶姻（一种生物基平台化学品）
影响因子：
9.8
作者：
Zhang, Lijie;Liu, Qiuyuan;Ma, Cuiqing
通讯作者：
Ma, Cuiqing
影响因子：
4.6
作者：
Bae SJ;Kim S;Hahn JS
通讯作者：
Hahn JS