Evolutionary story of a satellite DNA from Phodopus sungorus (Rodentia, Cricetidae).
Evolutionary story of a satellite DNA from Phodopus sungorus (Rodentia, Cricetidae).
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DOI：
10.1093/gbe/evu233
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发表时间：
2014-10-21
影响因子：
3.3
通讯作者：
Chaves R
中科院分区：
文献类型：
--
作者：
Paço A;Adega F;Meštrović N;Plohl M;Chaves R
为了有助于理解卫星 DNA 进化及其基因组参与，在这项工作中，从 Phodopus sungorus（仓鼠科）中分离并表征了第一个卫星 DNA (PSUcentSat)。 P. sungorus 中该序列的物理图谱显示大型 PSUcentSat 阵列位于五个常染色体对和 Y 染色体的异染色质（周围）着丝粒区域。其他仓鼠科和鼠科啮齿动物的基因组中也证实了直系同源 PSUcentSat 序列的存在，但呈现出散在的染色体分布。这种分布模式表明 PSUcentSat 分散在鼠科/仓鼠科的祖先中，后来假设，在 P.sungorus 的后代基因组中，限制性定位于（周围）着丝粒区域的少数染色体。我们认为，在所研究的物种分化之后，PSUcentSat 最有可能通过重组机制在该仓鼠的一些染色体对的着丝粒（周围）区域中高度扩增。花束染色体构型（前期 I）可能在这种选择性扩增中发挥重要作用，提供具有相似大小和/或形态的染色体之间着丝粒区域的物理接近性。这对于 P.sungorus 的近端着丝粒染色体（包括 Y 染色体）来说似乎尤其明显，所有染色体在着丝粒（周围）区域均呈现大型 PSUcentSat 阵列。该序列在所研究的基因组中的保守性及其在 P. sungorus 中的（周围）着丝粒扩增强烈表明了功能意义，可能在不同的基因组中显示了该卫星家族的不同功能。对正常增殖细胞中 PSUcentSat 转录活性的验证表明，其转录不受阶段限制，正如其他一些卫星所描述的那样。
With the goal to contribute for the understanding of satellite DNA evolution and its genomic involvement, in this work it was isolated and characterized the first satellite DNA (PSUcentSat) from Phodopus sungorus (Cricetidae). Physical mapping of this sequence in P. sungorus showed large PSUcentSat arrays located at the heterochromatic (peri)centromeric region of five autosomal pairs and Y-chromosome. The presence of orthologous PSUcentSat sequences in the genomes of other Cricetidae and Muridae rodents was also verified, presenting however, an interspersed chromosomal distribution. This distribution pattern suggests a PSUcentSat-scattered location in an ancestor of Muridae/Cricetidae families, that assumed afterwards, in the descendant genome of P. sungorus a restricted localization to few chromosomes in the (peri)centromeric region. We believe that after the divergence of the studied species, PSUcentSat was most probably highly amplified in the (peri)centromeric region of some chromosome pairs of this hamster by recombinational mechanisms. The bouquet chromosome configuration (prophase I) possibly displays an important role in this selective amplification, providing physical proximity of centromeric regions between chromosomes with similar size and/or morphology. This seems particularly evident for the acrocentric chromosomes of P. sungorus (including the Y-chromosome), all presenting large PSUcentSat arrays at the (peri)centromeric region. The conservation of this sequence in the studied genomes and its (peri)centromeric amplification in P. sungorus strongly suggests functional significance, possibly displaying this satellite family different functions in the different genomes. The verification of PSUcentSat transcriptional activity in normal proliferative cells suggests that its transcription is not stage-limited, as described for some other satellites.
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DOI：
10.1159/000337122
发表时间：
2012-01-01
期刊：
REPETITIVE DNA
影响因子：
--
作者：
Plohl, M.;Mestrovic, N.;Mravinac, B.
通讯作者：
Mravinac, B.
影响因子：
2.5
作者：
MODI, WS
通讯作者：
MODI, WS
影响因子：
3.4
作者：
Cazaux B;Catalan J;Veyrunes F;Douzery EJ;Britton-Davidian J
通讯作者：
Britton-Davidian J
影响因子：
1.6
作者：
MODI, WS
通讯作者：
MODI, WS
影响因子：
14.9
作者：
Benson, G
通讯作者：
Benson, G