A Systematic Study of RNAi Effects and dsRNA Stability in Tribolium castaneum and Acyrthosiphon pisum, Following Injection and Ingestion of Analogous dsRNAs.
A Systematic Study of RNAi Effects and dsRNA Stability in Tribolium castaneum and Acyrthosiphon pisum, Following Injection and Ingestion of Analogous dsRNAs.
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DOI：
10.3390/ijms19041079
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发表时间：
2018-04-04
影响因子：
5.6
通讯作者：
Fitches EC
中科院分区：
文献类型：
--
作者：
Cao M;Gatehouse JA;Fitches EC
RNA干扰(RNAi)在昆虫中的作用是高度可变的，可能在很大程度上取决于引入的双链RNA对核酸酶消化的稳定性。在这里，我们系统地比较了敏感的红粉甲虫(Tribolium Castaneum)和顽固的豌豆蚜虫(Acyrthosiphon Pisum)在靶向表达V型ATPase亚单位E(VTE)和凋亡抑制因子(IAP)基因的相同长度的dsRNAs后对RNAi的影响。注射和摄入VTE和IAP dsRNAs导致卡氏锥虫幼虫高达100%的死亡，并持续抑制(>；80%)转录水平。在豌豆根结线虫中，注射VTE而不是IAP dsRNA导致高达65%的死亡率和VTE转录水平的瞬时抑制(约40%)。以VTE dsRNA为食的蚜虫生长和繁殖力下降，尽管没有获得基因抑制的证据。蚜虫唾液、血淋巴和肠道核酸酶对dsRNAs的快速降解与卡氏粉虱幼虫的稳定性形成对比，后者似乎是外核酸酶活性导致dsRNAs消化相对较慢的原因。这是第一个直接比较RNAi效应和dsRNA稳定性的研究，并提供了进一步的证据，表明dsRNA对核酸酶的敏感性是决定RNAi效率的关键因素。
RNA interference (RNAi) effects in insects are highly variable and may be largely dependent upon the stability of introduced double-stranded RNAs to digestion by nucleases. Here, we report a systematic comparison of RNAi effects in susceptible red flour beetle (Tribolium castaneum) and recalcitrant pea aphid (Acyrthosiphon pisum) following delivery of dsRNAs of identical length targeting expression of V-type ATPase subunit E (VTE) and inhibitor of apoptosis (IAP) genes. Injection and ingestion of VTE and IAP dsRNAs resulted in up to 100% mortality of T. castaneum larvae and sustained suppression (>80%) of transcript levels. In A. pisum, injection of VTE but not IAP dsRNA resulted in up to 65% mortality and transient suppression (ca. 40%) of VTE transcript levels. Feeding aphids on VTE dsRNA reduced growth and fecundity although no evidence for gene suppression was obtained. Rapid degradation of dsRNAs by aphid salivary, haemolymph and gut nucleases contrasted with stability in T. castaneum larvae where it appears that exo-nuclease activity is responsible for relatively slow digestion of dsRNAs. This is the first study to directly compare RNAi effects and dsRNA stability in receptive and refractory insect species and provides further evidence that dsRNA susceptibility to nucleases is a key factor in determining RNAi efficiency.
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RNAi在豌豆蚜虫acyrthosiphon pisum中敲除基因。
影响因子：
3.5
作者：
Jaubert-Possamai, Stephanie;Le Trionnaire, Gael;Bonhomme, Joel;Christophides, Georges K;Rispe, Claude;Tagu, Denis
通讯作者：
Tagu, Denis
影响因子：
3.8
作者：
Arimatsu, Yuji;Kotani, Eiji;Furusawa, Toshiharu
通讯作者：
Furusawa, Toshiharu
影响因子：
2.2
作者：
Garbutt, Jennie S.;Belles, Xavier;Reynolds, Stuart E.
通讯作者：
Reynolds, Stuart E.
DOI：
10.1073/pnas.0504982102
发表时间：
2005-08-09
影响因子：
11.1
作者：
Arakane, Y;Muthukrishnan, S;Kramer, KJ
通讯作者：
Kramer, KJ
影响因子：
2.2
作者：
Allen, Margaret L.;Walker, William B., III
通讯作者：
Walker, William B., III