Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b regulates multiple developmental genes under benign and stress conditions.
Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b regulates multiple developmental genes under benign and stress conditions.
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DOI：
10.1093/jxb/ery142
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发表时间：
2018-05-19
影响因子：
6.9
通讯作者：
Mullineaux PM
中科院分区：
文献类型：
--
作者：
Albihlal WS;Obomighie I;Blein T;Persad R;Chernukhin I;Crespi M;Bechtold U;Mullineaux PM
在拟南芥（Arabidopsis thaliana）中，热休克转录因子a1b （HSFA1b）控制着对环境胁迫的抗性，并通过影响种子产量来决定生殖适应性。为了了解HSFA1b是如何实现这一目标的，我们调查了其全基因组靶点（ChIP-seq）及其在非胁迫（NS）、热胁迫（HS）下野生型和NS下HSFA1b过表达植物中转录组（RNA-seq）的影响。共鉴定出952个差异表达的hsfa1b靶向基因，其中至少85个与发育相关，主要在NS下结合。另有1780个基因差异表达，但不与HSFA1b结合，其中281个被归类为具有发育相关功能。这些基因通过27个转录因子（TFs）的分层网络间接调节。此外，我们鉴定了480个与HSFA1b结合的天然反义非编码RNA （cisNAT）基因，确定了进一步的间接调控模式。最后，hsfa1b靶向的基因组特征不仅包含热休克元件，还包含MADS box、LEAFY和G-Box启动子基序。这表明HSFA1b是针对一组常见的应激防御和发育基因的8个tf之一。我们提出HSFA1b将环境信号转导到许多耐受性和发育基因，使植物能够在不同的环境中持续调节其生长和发育。植物必须协调基因表达来平衡生长和逆境防御。拟南芥HSFA1b通过在热应激开始时重新配置一个广泛的转录调控网络来影响这一点。
In Arabidopsis thaliana, HEAT SHOCK TRANSCRIPTION FACTORA1b (HSFA1b) controls resistance to environmental stress and is a determinant of reproductive fitness by influencing seed yield. To understand how HSFA1b achieves this, we surveyed its genome-wide targets (ChIP-seq) and its impact on the transcriptome (RNA-seq) under non-stress (NS), heat stress (HS) in the wild type, and in HSFA1b-overexpressing plants under NS. A total of 952 differentially expressed HSFA1b-targeted genes were identified, of which at least 85 are development associated and were bound predominantly under NS. A further 1780 genes were differentially expressed but not bound by HSFA1b, of which 281 were classified as having development-associated functions. These genes are indirectly regulated through a hierarchical network of 27 transcription factors (TFs). Furthermore, we identified 480 natural antisense non-coding RNA (cisNAT) genes bound by HSFA1b, defining a further mode of indirect regulation. Finally, HSFA1b-targeted genomic features not only harboured heat shock elements, but also MADS box, LEAFY, and G-Box promoter motifs. This revealed that HSFA1b is one of eight TFs that target a common group of stress defence and developmental genes. We propose that HSFA1b transduces environmental cues to many stress tolerance and developmental genes to allow plants to adjust their growth and development continually in a varying environment. Plants must co-ordinate gene expression to balance growth and stress defence. Arabidopsis HSFA1b influences this by reconfiguring an extensive transcription regulatory network at the onset of heat stress.
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