Parallel, tag-directed assembly of locally derived short sequence reads.

Parallel, tag-directed assembly of locally derived short sequence reads.
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DOI:
10.1038/nmeth.1416
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发表时间:
2010-02
期刊:
影响因子:
48
通讯作者:
Shendure, Jay
Shendure, Jay
中科院分区:
生物学1区
文献类型:
--
作者:
Hiatt, Joseph B.;Patwardhan, Rupali P.;Turner, Emily H.;Lee, Choli;Shendure, Jay

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We demonstrate subassembly, anin vitrolibrary construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accuratede novogenome assembly and metagenome sequencing.
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