Parallel, tag-directed assembly of locally derived short sequence reads.
Parallel, tag-directed assembly of locally derived short sequence reads.
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DOI:
10.1038/nmeth.1416
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发表时间:
2010-02
期刊:
影响因子:
48
通讯作者:
Shendure, Jay
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文献类型:
--
作者:
Hiatt, Joseph B.;Patwardhan, Rupali P.;Turner, Emily H.;Lee, Choli;Shendure, Jay
We demonstrate subassembly, anin vitrolibrary construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accuratede novogenome assembly and metagenome sequencing.
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作者:
Bentley G;Higuchi R;Hoglund B;Goodridge D;Sayer D;Trachtenberg EA;Erlich HA
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DOI:
10.1126/science.1170020
发表时间:
2009-05-08
期刊:
Science (New York, N.Y.)
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