Yeast Irc22 Is a Novel Dsk2-Interacting Protein that Is Involved in Salt Tolerance.
Yeast Irc22 Is a Novel Dsk2-Interacting Protein that Is Involved in Salt Tolerance.
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DOI：
10.3390/cells3020180
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发表时间：
2014-03-27
期刊：
影响因子：
6
通讯作者：
Sekiguchi T
中科院分区：
文献类型：
--
作者：
Ishii T;Funakoshi M;Kobayashi H;Sekiguchi T
酵母泛素样和泛素相关蛋白Dsk 2是在泛素-蛋白酶体途径中起作用的泛素受体之一。我们筛选的Dsk 2相互作用的蛋白质在酿酒酵母的双杂交试验，并确定了一种新的Dsk 2相互作用的蛋白质，Irc 22，其基因位点先前已被描述为YEL 001 C，但其功能是未知的。IRC 22/YEL 001 C编码225个氨基酸残基，计算分子量为25 kDa。在酵母细胞中检测到Irc 22蛋白。IRC 22基因是酵母生长的非必需基因，在子囊菌酵母中发现了其同源基因。Irc 22在酵母细胞中与Dsk 2相互作用，但不与Rad 23和Ddi 1相互作用。通过过度表达或破坏IRC 22，泛素依赖性降解轻度受损。与野生型相比，dsk 2 Δ表现出盐敏感性，而irc 22 Δ表现出耐盐性。在IRC 22 Δ中观察到的耐盐表型在dsk 2 Δ IRC 22 Δ双破坏体中消失，表明DSK 2正性地参与盐胁迫耐受，而IRC 22负性地参与盐胁迫耐受。当比较irc 22 Δ和野生型菌株时，irc 22破坏不影响对DNA损伤和氧化应激的任何反应。总的来说，这些结果表明Dsk 2和Irc 22参与酵母的盐胁迫耐受性。
The yeast ubiquitin-like and ubiquitin-associated protein Dsk2 is one of the ubiquitin receptors that function in the ubiquitin-proteasome pathway. We screened the Dsk2-interacting proteins in Saccharomyces cerevisiae by a two-hybrid assay and identified a novel Dsk2-interacting protein, Irc22, the gene locus of which has previously been described as YEL001C, but the function of which is unknown. IRC22/YEL001C encodes 225 amino acid residues with a calculated molecular weight of 25 kDa. The Irc22 protein was detected in yeast cells. IRC22 was a nonessential gene for yeast growth, and its homologs were found among ascomycetous yeasts. Irc22 interacted with Dsk2 in yeast cells, but not with Rad23 and Ddi1. Ubiquitin-dependent degradation was impaired mildly by over-expression or disruption of IRC22. Compared with the wild-type strain, dsk2Δ exhibited salt sensitivity while irc22Δ exhibited salt tolerance at high temperatures. The salt-tolerant phenotype that was observed in irc22Δ disappeared in the dsk2Δirc22Δ double disruptant, indicating that DSK2 is positively and IRC22 is negatively involved in salt stress tolerance. IRC22 disruption did not affect any responses to DNA damage and oxidative stress when comparing the irc22Δ and wild-type strains. Collectively, these results suggest that Dsk2 and Irc22 are involved in salt stress tolerance in yeast.
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