Methylation-induced downregulation and tumor-suppressive role of microRNA-98 in glioma through targeting Sal-like protein 4.
Methylation-induced downregulation and tumor-suppressive role of microRNA-98 in glioma through targeting Sal-like protein 4.
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DOI：
10.3892/ijmm.2018.3464
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发表时间：
2018-05
影响因子：
5.4
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Xia Z;Qiu D;Deng J;Jiao X;Yang R;Sun Z;Wan X;Li J
MicroRNAs (miRs) have been found to play key roles in various human cancers, but the detailed regulatory mechanism of miR-98 in glioma remains largely unknown. The findings of the present study demonstrated that miR-98 was frequently downregulated in glioma tissues and cell lines (U87, U251, U373 and SHG44), and the decreased miR-98 levels were associated with DNA methylation. Treatment with 5-Aza-20-deoxycytidine, a DNA methyltransferase inhibitor, significantly increased the expression of miR-98 in glioma cells. Moreover, both miR-98 downregulation and methylation were significantly associated with a more aggressive tumor phenotype in glioma, as well as shorter survival time of glioma patients. Restoration of miR-98 expression caused a marked decrease in the migration and invasion of U87 cells, but did not affect cell proliferation. Sal-like protein 4 (SALL4) was further identified as a novel target gene of miR-98, and its protein expression was negatively regulated by miR-98 in U87 cells. Restoration of SALL4 expression reversed the suppressive effects of miR-98 on the migration and invasion of U87 cells. Furthermore, SALL4 was significantly upregulated in glioma tissues and cell lines, and an inverse correlation between miR-98 and SALL4 expression in glioma tissues was identified. In addition, the increased expression of SALL4 was significantly associated with glioma progression. Taken together, these data demonstrated that downregulation of miR-98, induced by methylation, promotes glioma cell migration and invasion via targeting SALL4. Therefore, miR-98 may become a potential therapeutic candidate for glioma.
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影响因子：
--
作者：
Chen Z;Cheng Q;Ma Z;Xi H;Peng R;Jiang B
通讯作者：
Jiang B
影响因子：
--
作者：
Deng G;Zhu L;Huang F;Nie W;Huang W;Xu H;Zheng S;Yi Z;Wan T
通讯作者：
Wan T
影响因子：
9.8
作者：
John B;Enright AJ;Aravin A;Tuschl T;Sander C;Marks DS
通讯作者：
Marks DS
影响因子：
3.2
作者：
Ashby LS;Smith KA;Stea B
通讯作者：
Stea B
PI3K抑制剂与miR-125b抑制剂联合通过灭活Wnt/β-catenin信号通路使TMZ诱导的抗神经胶质瘤干癌作用变得敏感
DOI：
10.1007/s11626-015-9931-x
发表时间：
2015-11-01
影响因子：
2.1
作者：
Shi, Lei;Fei, Xifeng;You, Yongping
通讯作者：
You, Yongping