High-resolution multi-marker DNA metabarcoding reveals sexual dietary differentiation in a bird with minor dimorphism.
High-resolution multi-marker DNA metabarcoding reveals sexual dietary differentiation in a bird with minor dimorphism.
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DOI：
10.1002/ece3.6687
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发表时间：
2020-10
影响因子：
2.6
通讯作者：
Beja P
中科院分区：
文献类型：
--
作者：
da Silva LP;Mata VA;Lopes PB;Lopes RJ;Beja P
虽然鸟类的性别饮食差异是众所周知的，但它通常与雄性和雌性之间显著的形态二态性有关，在两性单一性或只有轻微二态性的物种中，分化程度较低。然而，这可能是在大多数传统饮食研究中取得的分类分辨率差的产物，这可能无法检测猎物消耗的微妙种内差异。在这里，我们展示了多标记元条形码解决这些问题的能力，重点关注一种稍微二态的通才雀形目，黑麦穗Oenanthe leucura。利用来自4个基因组区域（18S、16S、COI和trnL）的标记，我们分析了在繁殖季节收集的93个成年黑麦粪便。我们发现两性在喙和身体特征上相当相似，尽管雄性的喙略厚，翅膀和尾巴比雌性长。雌雄蚁均以广泛的节肢动物种类和少量肉质水果为主，但总体上雄性蚁的食性多样性高于雌性蚁，且雌性蚁的食性发生率（58%）远高于雄性蚁（29%）。我们假设观察到的性别分化可能与雌性觅食更接近其后代的丰富猎物有关，而雄性在觅食范围更广的同时消耗更多种类的猎物。总之，我们的研究结果表明，鸟类的饮食性别分化可能比目前认识到的更为普遍，而多标记DNA元条形码是揭示这种差异的一个特别强大的工具。研究发现，黑麦雄性和雌性捕食的猎物不同，雄性的猎物多样性更高，雌性捕食蚂蚁的频率更高。这些模式将无法被显示较低分类学分辨率的技术检测到，从而突出了元条形码在揭示新的生态模式方面的潜力。
Although sexual dietary differentiation is well known in birds, it is usually linked with significant morphological dimorphism between males and females, with lower differentiation reported in sexually monomorphic or only slightly dimorphic species. However, this may be an artifact of poor taxonomic resolution achieved in most conventional dietary studies, which may be unable to detect subtle intraspecific differentiation in prey consumption. Here, we show the power of multi‐marker metabarcoding to address these issues, focusing on a slightly dimorphic generalist passerine, the black wheatear Oenanthe leucura. Using markers from four genomic regions (18S, 16S, COI, and trnL), we analyzed fecal droppings collected from 93 adult black wheatears during the breeding season. We found that sexes were rather similar in bill and body features, though males had a slightly thicker bill and longer wings and tail than females. Diet was dominated in both sexes by a very wide range of arthropod species and a few fleshy fruits, but the overall diet diversity was higher for males than females, and there was a much higher frequency of occurrence of ants in female (58%) than male (29%) diets. We hypothesize that the observed sexual differentiation was likely related to females foraging closer to their offspring on abundant prey, while males consumed a wider variety of prey while foraging more widely. Overall, our results suggest that dietary sexual differentiation in birds may be more widespread than recognized at present and that multi‐marker DNA metabarcoding is a particularly powerful tool to unveiling such differences. We found that males and females of Black wheatears are eating different prey, with males showing a higher prey diversity and females preying more often on ants. These patterns would be undetected with techniques showing lower taxonomic resolution, thus highlighting the potential of metabarcoding in revealing novel ecological patterns.
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影响因子：
4.6
作者：
Alarcón PAE;Morales JM;Donázar JA;Sánchez-Zapata JA;Hiraldo F;Lambertucci SA
通讯作者：
Lambertucci SA
影响因子：
7.7
作者：
da Silva, Luis P.;Mata, Vanessa A.;Beja, Pedro
通讯作者：
Beja, Pedro
影响因子：
2.4
作者：
Elliott, Kyle Hamish;Gaston, Anthony J.;Crump, Douglas
通讯作者：
Crump, Douglas
影响因子：
4.8
作者：
Chao, Anne;Jost, Lou
通讯作者：
Jost, Lou
影响因子：
7.7
作者：
Brown, David S.;Jarman, Simon N.;Symondson, William O. C.
通讯作者：
Symondson, William O. C.