Yeast surface display for protein engineering and characterization.
Yeast surface display for protein engineering and characterization.
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DOI:
10.1016/j.sbi.2007.08.012
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发表时间:
2007-08
影响因子:
6.8
通讯作者:
Wittrup, K. Dane
中科院分区:
文献类型:
--
作者:
Gai, S. Annie;Wittrup, K. Dane
Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein–protein interactions, represents a significant recent advance.
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