Specific induction of self‐discrimination by follicle cells in Ciona intestinalis oocytes
Specific induction of self‐discrimination by follicle cells in Ciona intestinalis oocytes
复制标题
玻璃海鞘卵母细胞中滤泡细胞特异性诱导自我歧视
DOI：
10.1046/j.1440-169x.1995.t01-2-00006.x
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发表时间：
1995
期刊：
影响因子：
4.6
通讯作者：
N. Usui
中科院分区：
文献类型：
--
作者：
M. Pinto;R. Santis;R. Marino;N. Usui
自交不亲和性是一种阻止海鞘自体受精的机制，其基础是卵母细胞卵黄膜仅区分和接受异源精子的能力。在玻璃海鞘中，自我歧视是在卵子发生晚期建立的，并由上层滤泡细胞的产物贡献或控制。在这项研究中，我们通过使用去除卵泡细胞并与自体或异源卵泡细胞一起孵育的卵巢卵母细胞的体外成熟，进一步研究了卵泡细胞在自我歧视发生中的作用。受精试验表明，即使卵泡细胞与卵黄膜分离，卵泡细胞仍能发挥作用，并且只有自体卵泡细胞才能促进卵膜自体不育的诱导。成熟过程中卵母细胞的电子显微镜显示，从自体受精到自体不育的转变伴随着卵黄层外表面出现薄薄的电子致密层。我们认为该层的形成是卵泡细胞产物与自体卵黄层相互作用的结果。
Self‐incompatibility, a mechanism that prevents self‐fertilization in ascidians, is based on the ability of the oocyte vitelline coat to distinguish and accept only heterologous spermatozoa. In Ciona intestinalis self‐discrimination is established during late oogenesis and is contributed or controlled by products of the overlying follicle cells. In this study we have further investigated the role of the follicle cells in the onset of self‐discrimination by using in vitro maturation of ovarian oocytes deprived of the follicle cells and incubated with either autologous or heterologous follicle cells. Fertilization assays demonstrate that the action of the follicle cells is exerted even when they are detached from the vitelline coat and that only autologous follicle cells can promote the induction of self‐sterility on the egg coat. Electron microscopy of the oocytes during maturation reveals that the switch from self‐fertility to self‐sterility is accompanied by the appearance of a thin electron‐dense layer on the outer surface of the vitelline coat. We suggest that the formation of this layer is the result of the interaction between products of the follicle cells and the autologous vitelline coat.
影响因子：
64.8
作者：
SCOFIELD, VL;SCHLUMPBERGER, JM;WEISSMAN, IL
通讯作者：
WEISSMAN, IL