Inflammatory phenotype modulation in the respiratory tract and systemic circulation of e-cigarette users: a pilot study.
Inflammatory phenotype modulation in the respiratory tract and systemic circulation of e-cigarette users: a pilot study.
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电子烟使用者呼吸道和体循环的炎症表型调节：一项试点研究。
DOI：
10.1152/ajplung.00363.2021
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
CrottyAlexander,LauraE
中科院分区：
文献类型：
--
作者：
Sayed,IbrahimM;Masso-Silva,JorgeA;Mittal,Ankita;Patel,Arjun;Lin,Erica;Moshensky,Alex;Shin,John;Bojanowski,ChristineM;Das,Soumita;Akuthota,Praveen;CrottyAlexander,LauraE
全世界有超过4000万人使用电子烟，但长期使用电子烟对健康的影响尚未得到充分界定。特别是，电子烟气雾吸入对全身炎症和宿主防御的影响尚未完全了解。我们进行了一项纵向队列试点研究，以探索电子烟使用者（n= 20）与健康对照组（n= 13）的炎症状态和单核细胞功能的变化，并评估减少电子烟使用对炎症状态和单核细胞功能的影响。唾液，痰液和血液是从基线时的电子烟使用者和减少电子烟使用的2周干预后获得的。总体而言，在通过多重量化的38种蛋白质中，来自电子烟使用者的气道样本相对于健康对照组的免疫调节蛋白水平往往降低，而循环中的细胞因子，趋化因子和生长因子水平往往升高。具体而言，电子烟使用者唾液中IL-1受体拮抗剂（IL-1 Ra）水平较低（P< 0.0001），痰液中IL-1 Ra和生长调节癌基因（GRO）水平较高（分别为P< 0.01和P < 0.05），血浆中TNFβ（P< 0.0001）和VEGF（P< 0.0001）水平较高。来自电子烟使用者的循环单核细胞响应于减少电子烟使用而改变了其炎症表型，在用细菌脂多糖攻击时，IL-8和IL-6释放减弱（分别为P< 0.001和P < 0.05），表明对细菌感染适当反应的能力降低。基于这些发现，长期吸入电子烟气溶胶会改变气道和体循环的炎症状态，引起人们对电子烟长期使用者炎症和传染病发展的担忧。
Over 40 million people use e-cigarettes worldwide, but the impact of chronic e-cigarette use on health has not been adequately defined. In particular, effects of e-cigarette aerosol inhalation on inflammation and host defenses across the body are not fully understood. We conducted a longitudinal cohort pilot study to explore changes in the inflammatory state and monocyte function of e-cigarette users (n= 20) versus healthy controls (n= 13) and to evaluate effects of e-cigarette use reduction on the same. Saliva, sputum, and blood were obtained from e-cigarette users at baseline and after a 2-wk intervention of decreased e-cigarette use. Overall, across 38 proteins quantified by multiplex, airway samples from e-cigarette users tended to have decreased levels of immunomodulatory proteins relative to healthy controls, whereas levels of cytokines, chemokines, and growth factors in the circulation tended to be elevated. Specifically, e-cigarette users had lower levels of IL-1 receptor antagonist (IL-1Ra) in saliva (P< 0.0001), with higher IL-1Ra and growth-regulated oncogene (GRO) levels in sputum (P< 0.01 andP< 0.05, respectively), and higher levels of both TNFβ (P< 0.0001) and VEGF (P< 0.0001) in plasma. Circulating monocytes from e-cigarette users had alterations in their inflammatory phenotype in response to reduced e-cigarette use, with blunted IL-8 and IL-6 release upon challenge with bacterial lipopolysaccharide (P< 0.001 andP< 0.05, respectively), suggesting a decreased ability to appropriately respond to bacterial infection. Based on these findings, chronic inhalation of e-cigarette aerosols alters the inflammatory state of the airways and systemic circulation, raising concern for the development of both inflammatory and infectious diseases in chronic users of e-cigarettes.
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影响因子：
5.8
作者：
Zanini A;Chetta A;Imperatori AS;Spanevello A;Olivieri D
通讯作者：
Olivieri D
影响因子：
6.4
作者：
Nielsen, Marlene C.;Andersen, Morten N.;Moller, Holger J.
通讯作者：
Moller, Holger J.
DOI：
10.1152/ajpregu.00270.2017
发表时间：
2018-06-01
影响因子：
2.8
作者：
Alexander, Laura E. Crotty;Drummond, Christopher A.;Breen, Ellen C.
通讯作者：
Breen, Ellen C.
影响因子：
6.7
作者：
Rawlinson, A;Dalati, MHN;Fairclough, AL
通讯作者：
Fairclough, AL