Optimization of manganese peroxidase production by the white rot fungus Bjerkandera sp. strain BOS55
Optimization of manganese peroxidase production by the white rot fungus Bjerkandera sp. strain BOS55
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白腐真菌 Bjerkandera sp 生产锰过氧化物酶的优化。
DOI：
10.1111/j.1574-6968.1997.tb13873.x
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发表时间：
1997
影响因子：
2.1
通讯作者：
J. Field
中科院分区：
文献类型：
--
作者：
T. Mester;J. Field
锰依赖过氧化物酶(MNP)是白腐菌产生的最普遍的过氧化物酶。众所周知，MNP参与木质素降解、生物漂白和有害有机污染物的氧化。BJerkandera sp.菌株BOS55是一种氮素不受调控的白腐菌，由于生物产量的增加，它在氮素营养过剩的情况下产生大量的MNP。因此，该菌株是大规模生产该酶的良好候选菌株。本研究的目的是通过改变不同的生理因素，如锰浓度、培养pH、培养温度和有机酸的添加，来优化氮充足培养条件下的MNP产量。当锰浓度为0.2~1 mM、pH值为5.2、培养温度为30℃时，该菌产锰能力最强(可达90 0U 1−1)。添加乙醇酸、丙二酸、葡萄糖醛酸、葡萄糖酸、2-羟基丁酸等有机酸，可使菌体中锰磷酸峰滴度提高到1250U1−1。
Manganese dependent peroxidase (MnP) is the most ubiquitous peroxidase produced by white rot fungi. MnP is known to be involved in lignin degradation, biobleaching and in the oxidation of hazardous organopollutants. Bjerkandera sp. strain BOS55 is a nitrogen-unregulated white rot fungus which produces high amounts of MnP in the excess of N-nutrients due to increased biomass yield. Therefore, the strain is a good candidate for use in large scale production of this enzyme. The objective of this study was to optimize the MnP production in N-sufficient cultures by varying different physiological factors such as Mn concentration, culture pH, incubation temperature and the addition of organic acids. The fungus produced the highest level of MnP (up to 900 U 1−1) when the Mn concentration was 0.2 to 1 mM, the pH value was 5.2, and the incubation temperature was 30°C. A noteworthy finding was that MnP was also produced at lower levels in the complete absence of Mn. The addition of organic acids like glycolate, malonate, glucuronate, gluconate, 2-hydroxybutyrate to the culture medium increased the peak titres of MnP up to 1250 U 1−1. FPLC profiles indicated that the organic acids stimulated the production of all MnP isoenzymes present in the extracellular fluid of the fungus.
DOI：
10.1073/pnas.90.4.1242
发表时间：
1993-02-15
影响因子：
11.1
作者：
KUAN, IC;TIEN, M
通讯作者：
TIEN, M
木质素过氧化物酶也可以氧化锰。
影响因子：
2.9
作者：
Khindaria,A;Barr,DP;Aust,SD
通讯作者：
Aust,SD