Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.
Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.
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DOI：
10.3390/toxins6041222
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发表时间：
2014-03-28
期刊：
影响因子：
4.2
通讯作者：
Ben-Dov E
中科院分区：
文献类型：
--
作者：
Ben-Dov E
苏云金芽孢杆菌亚种以色列芽孢杆菌（Bacillus thuringiensis, Bti）是世界上首次发现的苏云金芽孢杆菌，是一种有效的防蚊剂。其杀虫活性存在于由cry4Aa、cry4Ba、cry11Aa、cyt1Aa、cry10Aa和cyt2Ba编码的4个主要多肽（134、128、72和27 kDa）和至少2个次要多肽（78和29 kDa）中，这些多肽都被定位在128 kb的pBtoxis质粒上。这6种δ内毒素对伊蚊、库蚊和按蚊幼虫具有高度、特异性和不同的毒性，形成复杂的副孢子结晶体。Cry毒素由三个结构域（穿孔结构域I和受体结合II和III）组成，并产生阳离子选择性通道，而Cyts由一个结构域组成，它可以起到类似洗涤剂的膜穿孔剂的作用。尽管单独使用Cyt1Aa和Cyt2Ba对暴露的幼虫的毒性较低，但它们与Cry毒素具有高度协同作用，因此它们的组合可以防止靶标中出现抗性。在使用bti生物杀虫剂治疗了几十年的野外蚊子种群中缺乏明显的耐药性，这表明这种细菌在未来几年将是一种有效的生物防治剂。
Bacillus thuringiensis subsp. israelensis (Bti) is the first Bacillus thuringiensis to be found and used as an effective biological control agent against larvae of many mosquito and black fly species around the world. Its larvicidal activity resides in four major (of 134, 128, 72 and 27 kDa) and at least two minor (of 78 and 29 kDa) polypeptides encoded respectively by cry4Aa, cry4Ba, cry11Aa, cyt1Aa, cry10Aa and cyt2Ba, all mapped on the 128 kb plasmid known as pBtoxis. These six δ-endotoxins form a complex parasporal crystalline body with remarkably high, specific and different toxicities to Aedes, Culex and Anopheles larvae. Cry toxins are composed of three domains (perforating domain I and receptor binding II and III) and create cation-selective channels, whereas Cyts are composed of one domain that acts as well as a detergent-like membrane perforator. Despite the low toxicities of Cyt1Aa and Cyt2Ba alone against exposed larvae, they are highly synergistic with the Cry toxins and hence their combinations prevent emergence of resistance in the targets. The lack of significant levels of resistance in field mosquito populations treated for decades with Bti-bioinsecticide suggests that this bacterium will be an effective biocontrol agent for years to come.
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影响因子：
4.4
作者：
BenDov, E;Einav, M;Zaritsky, A
通讯作者：
Zaritsky, A
影响因子：
3.2
作者：
BENDOV, E;BOUSSIBA, S;ZARITSKY, A
通讯作者：
ZARITSKY, A
影响因子：
3.3
作者：
Boussiba, S;Wu, XQ;Zaritsky, A
通讯作者：
Zaritsky, A
影响因子：
2.1
作者：
ANGSUTHANASOMBAT, C;CRICKMORE, N;ELLAR, DJ
通讯作者：
ELLAR, DJ
影响因子：
3.4
作者：
ALY, C;MULLA, MS;FEDERICI, BA
通讯作者：
FEDERICI, BA