Age-related hearing loss in Mn-SOD heterozygous knockout mice.
Age-related hearing loss in Mn-SOD heterozygous knockout mice.
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DOI：
10.1155/2013/325702
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发表时间：
2013
影响因子：
--
通讯作者：
Yamasoba T
中科院分区：
文献类型：
--
作者：
Kinoshita M;Sakamoto T;Kashio A;Shimizu T;Yamasoba T
听力损失（阿勒）降低了许多老年人的生活质量。锰超氧化物歧化酶（Mn-SOD）是线粒体内的抗氧化酶之一，在清除活性氧（ROS）方面起着至关重要的作用。为了确定Mn-SOD的减少是否加速阿勒，我们通过听觉脑干反应（ABR）评估了Mn-SOD杂合基因敲除（HET）小鼠及其同窝野生型（WT）C57 BL/6小鼠的听觉功能。平均ABR阈值显着增加，在16个月时相比，在4个月的WT和HET小鼠，但他们之间没有显着差异，在任何年龄。毛细胞损失的程度，螺旋神经节细胞密度，和血管纹的厚度也没有不同的WT和HET小鼠在任何年龄。16个月时，HET小鼠的SGC和SV中8-羟基脱氧鸟苷的免疫反应性显著高于WT小鼠，但4-羟基壬烯醛的免疫反应性在它们之间没有差异。这些结果表明，虽然Mn-SOD减少一半可能会在一定程度上增加耳蜗的氧化应激，但它可能不足以加速生理老化过程中与年龄相关的耳蜗损伤。
Age-related hearing loss (AHL) reduces the quality of life for many elderly individuals. Manganese superoxide dismutase (Mn-SOD), one of the antioxidant enzymes acting within the mitochondria, plays a crucial role in scavenging reactive oxygen species (ROS). To determine whether reduction in Mn-SOD accelerates AHL, we evaluated auditory function in Mn-SOD heterozygous knockout (HET) mice and their littermate wild-type (WT) C57BL/6 mice by means of auditory brainstem response (ABR). Mean ABR thresholds were significantly increased at 16 months when compared to those at 4 months in both WT and HET mice, but they did not significantly differ between them at either age. The extent of hair cell loss, spiral ganglion cell density, and thickness of the stria vascularis also did not differ between WT and HET mice at either age. At 16 months, immunoreactivity of 8-hydroxydeoxyguanosine was significantly greater in the SGC and SV in HET mice compared to WT mice, but that of 4-hydroxynonenal did not differ between them. These findings suggest that, although decrease of Mn-SOD by half may increase oxidative stress in the cochlea to some extent, it may not be sufficient to accelerate age-related cochlear damage under physiological aging process.
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