Optimizing two-phase system by experiment and simulation for high-speed counter-current chromatographic separation of 2-phenylbutyric acid enantiomer
Optimizing two-phase system by experiment and simulation for high-speed counter-current chromatographic separation of 2-phenylbutyric acid enantiomer
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通过实验和模拟优化高速逆流色谱分离2-苯基丁酸对映体的两相体系
DOI：
10.1080/01496395.2017.1283329
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发表时间：
2017-02
影响因子：
2.8
通讯作者：
Liu Yu
中科院分区：
文献类型：
--
作者：
Zhang Panliang;Xie Xiaojuan;Tang Kewen;Xu Weifeng;Xiong Biquan;Liu Yu
摘要提出了2-苯基丁酸(2-PBA)的高速逆流色谱制备分离方法。两相溶剂体系为正己烷/乙酸丁酯/水相(7：3：10，v/v/v)。考察了影响手性分离效率的因素(如手性选择剂(CS)的种类和浓度、水相的pH值和温度)。建立了数学模型，并进行了实验验证。结果表明，模型预测与实验结果吻合较好。在最佳条件下，2-PBA得到成功分离。单体纯度达到99.5%，对映体回收率为91-93%。
ABSTRACT High-speed counter-current chromatographic preparative separation of 2-phenylbutyric acid (2-PBA) was successfully proposed. The two-phase solvent system was composed of n-hexane/butyl acetate/aqueous phase (7:3:10, v/v/v). Factors influencing the chiral separation efficiency (e.g. types and concentrations of chiral selector (CS), pH of aqueous phase and temperature) were investigated. A mathematical model was established and verified by conducting experiments. Results showed that the model predictions were in good agreement with the experimental results. Under optimal conditions, 2-PBA was successfully separated. The monomer purity reached 99.5%, and the recovery of enantiomers was 91–93%.
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DOI：
10.2174/22132406113099990001
发表时间：
2014
期刊：
Current chromatography
影响因子：
--
作者：
Ma Y;Ito Y
通讯作者：
Ito Y
影响因子：
1.8
作者：
B. Shekunov;P. York
通讯作者：
B. Shekunov;P. York
影响因子：
1.3
作者：
Shengqiang Tong;Hu Zhang;Jizhong Yan
通讯作者：
Shengqiang Tong;Hu Zhang;Jizhong Yan
影响因子：
5.4
作者：
W. Kiridena;C. Poole;M. I. Nawas;W. W. Koziol-W.
通讯作者：
W. Kiridena;C. Poole;M. I. Nawas;W. W. Koziol-W.
影响因子：
--
作者：
Liya Rong;Qi Liu;Jun Wang;Hualiang Zeng;Hua Yang;Xiaoqing Chen
通讯作者：
Liya Rong;Qi Liu;Jun Wang;Hualiang Zeng;Hua Yang;Xiaoqing Chen