Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein.
Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein.
复制标题
表面增强共振拉曼散射免疫分析平台检测痕量SARS-CoV-2刺突蛋白。
DOI：
10.1016/j.talanta.2022.123381
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发表时间：
2022-07-01
期刊：
影响因子：
6.1
通讯作者：
Aoki PHB
中科院分区：
文献类型：
--
作者：
Bistaffa MJ;Camacho SA;Pazin WM;Constantino CJL;Oliveira ON Jr;Aoki PHB
冠状病毒病(新冠肺炎)的早期诊断需要准确检测遗传物质或敏感检测病毒蛋白。在这项工作中，我们设计了一个用于检测痕量SARS-CoV-2刺突蛋白(S)的免疫分析平台。它基于吸附在球形金纳米颗粒(AuNPs)上的亚甲基蓝(MB)的表面增强共振拉曼散射(SERRS)，并覆盖了6 nm的二氧化硅外壳。SerRS纳米探针中的后一个外壳阻止了与SARS-CoV-2抗体的聚集和功能化。免疫分析的特异性是通过将这种功能化与抗体固定在作为平台支撑的盖玻片上相结合来实现的。用多维投影技术Sammon‘s映射对SerRS数据进行处理，可以区分不同浓度的SARS-CoV-2抗原，证实干扰物质的缺乏。使用633nmSERRS，最低检测浓度为10pg/m L，检出限为0.046 ng/m L(0.60Pm)。这一值与新冠肺炎患者出现症状时的最低血浆浓度相当，表明SERRS免疫分析平台可用于早期诊断。
The early diagnosis of Coronavirus disease (COVID-19) requires either an accurate detection of genetic material or a sensitive detection of viral proteins. In this work, we designed an immunoassay platform for detecting trace levels of SARS-CoV-2 spike (S) protein. It is based on surface-enhanced resonance Raman scattering (SERRS) of methylene blue (MB) adsorbed onto spherical gold nanoparticles (AuNPs) and coated with a 6 nm silica shell. The latter shell in the SERRS nanoprobe prevented aggregation and permitted functionalization with SARS-CoV-2 antibodies. Specificity of the immunoassay was achieved by combining this functionalization with antibody immobilization on the cover slides that served as the platform support. Different concentrations of SARS-CoV-2 antigen could be distinguished and the lack of influence of interferents was confirmed by treating SERRS data with the multidimensional projection technique Sammon's mapping. With SERRS using a laser line at 633 nm, the lowest concentration of spike protein detected was 10 pg/mL, achieving a limit of detection (LOD) of 0.046 ng/mL (0.60 pM). This value is comparable to the lowest concentrations in the plasma of COVID-19 patients at the onset of symptoms, thus indicating that the SERRS immunoassay platform may be employed for early diagnosis.
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DOI：
10.1016/j.snb.2022.131415
发表时间：
2022-04-15
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Chen PH;Huang CC;Wu CC;Chen PH;Tripathi A;Wang YL
通讯作者：
Wang YL
影响因子：
3.5
作者：
Camacho, Sabrina A.;Aoki, Pedro H. B.;Constantino, Carlos J. L.
通讯作者：
Constantino, Carlos J. L.
影响因子：
9.9
作者：
Kamal, Surabhi;Yang, Thomas Chung-Kuang
通讯作者：
Yang, Thomas Chung-Kuang
影响因子：
2.5
作者：
Furini, Leonardo Negri;Sanchez-Cortes, Santiago;Leopoldo Constantino, Carlos Jose
通讯作者：
Leopoldo Constantino, Carlos Jose
影响因子：
56.9
作者：
AMIT, AG;MARIUZZA, RA;POLJAK, RJ
通讯作者：
POLJAK, RJ