Saliva-based COVID-19 detection: A rapid antigen test of SARS-CoV-2 nucleocapsid protein using an electrical-double-layer gated field-effect transistor-based biosensing system.
Saliva-based COVID-19 detection: A rapid antigen test of SARS-CoV-2 nucleocapsid protein using an electrical-double-layer gated field-effect transistor-based biosensing system.
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DOI：
10.1016/j.snb.2022.131415
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发表时间：
2022-04-15
期刊：
影响因子：
--
通讯作者：
Wang YL
中科院分区：
文献类型：
--
作者：
Chen PH;Huang CC;Wu CC;Chen PH;Tripathi A;Wang YL
面对新冠肺炎的不断涌动，诊断检测能力不足危及疾病传播的控制。作为病毒检测的金标准，实时逆转录聚合酶链式反应(Real-Time RT-PCR，Real-Time RT-PCR)由于设置集中、周转时间长，在紧急暴发期间未能及时反映疫情现状。因此，需要一个快速筛查工具来帮助遏制新冠肺炎在疫苗实施尚未广泛部署的国家中的传播。在这项工作中，我们提出了一种基于唾液的新冠肺炎抗原检测方法，它使用了双电层栅场效应晶体管生物传感器(BioFET)。1×PBS和人工唾液中SARS-CoV-2核衣壳(N)蛋白的检测下限分别为0.34 ng/mL(7.44 pm)和0.14 ng/mL(2.96 pm)。用MERS冠状病毒、甲型流感病毒和乙型流感病毒之间表现出低交叉反应的抗原类型来检验其特异性。这一便携式系统嵌入了蓝牙通信和用户友好的界面，完全兼容数字健康，可行地实现了现场扭亏为盈，有效的管理，以及医疗提供者和一线卫生工作者采取的积极应对措施。
Facing the unstopped surges of COVID-19, an insufficient capacity of diagnostic testing jeopardizes the control of disease spread. Due to a centralized setting and a long turnaround, real-time reverse transcription polymerase chain reaction (real-time RT-PCR), the gold standard of viral detection, has fallen short in timely reflecting the epidemic status quo during an urgent outbreak. As such, a rapid screening tool is necessitated to help contain the spread of COVID-19 amid the countries where the vaccine implementations have not been widely deployed. In this work, we propose a saliva-based COVID-19 antigen test using the electrical double layer (EDL)-gated field-effect transistor-based biosensor (BioFET). The detection of SARS-CoV-2 nucleocapsid (N) protein is validated with limits of detection (LoDs) of 0.34 ng/mL (7.44 pM) and 0.14 ng/mL (2.96 pM) in 1× PBS and artificial saliva, respectively. The specificity is inspected with types of antigens, exhibiting low cross-reactivity among MERS-CoV, Influenza A virus, and Influenza B virus. This portable system is embedded with Bluetooth communication and user-friendly interfaces that are fully compatible with digital health, feasibly leading to an on-site turnaround, an effective management, and a proactive response taken by medical providers and frontline health workers.
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影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
9.4
作者：
Lefever, Stefanie;Indevuyst, Christophe;Vermeersch, Pieter
通讯作者：
Vermeersch, Pieter
DOI：
10.1093/cid/ciaa1382
发表时间：
2021-11-02
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Arnaout R;Lee RA;Lee GR;Callahan C;Cheng A;Yen CF;Smith KP;Arora R;Kirby JE
通讯作者：
Kirby JE
影响因子：
4.6
作者：
Chu CH;Sarangadharan I;Regmi A;Chen YW;Hsu CP;Chang WH;Lee GY;Chyi JI;Chen CC;Shiesh SC;Lee GB;Wang YL
通讯作者：
Wang YL
影响因子：
3.7
作者：
Arevalo-Rodriguez I;Buitrago-Garcia D;Simancas-Racines D;Zambrano-Achig P;Del Campo R;Ciapponi A;Sued O;Martinez-García L;Rutjes AW;Low N;Bossuyt PM;Perez-Molina JA;Zamora J
通讯作者：
Zamora J