Clearance and phosphorylation of alpha-synuclein are inhibited in methionine sulfoxide reductase a null yeast cells.
Clearance and phosphorylation of alpha-synuclein are inhibited in methionine sulfoxide reductase a null yeast cells.
复制标题
DOI：
10.1007/s12031-009-9274-8
复制
发表时间：
2009-11
影响因子：
3.1
通讯作者：
Moskovitz, Jackob
中科院分区：
文献类型：
--
作者：
Oien, Derek B.;Shinogle, Heather E.;Moore, David S.;Moskovitz, Jackob
关键词：
聚集的α-突触核蛋白及其Ala 30 Pro和Ala 53 Thr点突变与帕金森病相关α-突触核蛋白和甲硫氨酸氧化的α-突触核蛋白的蛋白质结构和功能的生理作用尚未完全了解。甲硫氨酸亚砜还原酶A（MsrA）可还原甲硫氨酸亚砜残基，并作为抗氧化剂发挥作用。为了监测甲硫氨酸氧化为α-突触核蛋白对基本细胞过程的影响，在msrA无效突变体和野生型酵母细胞中表达α-突触核蛋白。与表达α-突触核蛋白的野生型细胞相比，表达α-突触核蛋白的msrA无效突变体细胞中的蛋白质降解受到抑制。在表达SynA 30 P的msrA无效突变细胞中观察到对降解的抑制增加和原纤化蛋白的积累增加。此外，蛋氨酸氧化抑制α-突触核蛋白在酵母细胞和酪蛋白激酶2体外磷酸化。因此，受损的MsrA功能结合α-突触核蛋白过表达可能促进导致突触核蛋白病的过程。
Aggregated α-synuclein and the point mutations Ala30Pro and Ala53Thr of α-synuclein are associated with Parkinson’s disease. The physiological roles of α-synuclein and methionine oxidation of the α-synuclein protein structure and function are not fully understood. Methionine sulfoxide reductase A (MsrA) reduces methionine sulfoxide residues and functions as an antioxidant. To monitor the effect of methionine oxidation to α-synuclein on basic cellular processes, α-synucleins were expressed in msrA null mutant and wild-type yeast cells. Protein degradation was inhibited in the α-synuclein-expressing msrA null mutant cells compared to α-synuclein-expressing wild-type cells. Increased inhibition of degradation and elevated accumulations of fibrillated proteins were observed in SynA30P-expressing msrA null mutant cells. Additionally, methionine oxidation inhibited α-synuclein phosphorylation in yeast cells and in vitro by casein kinase 2. Thus, a compromised MsrA function combined with α-synuclein overexpression may promote processes leading to synucleinopathies.
登录
查看更多内容
影响因子：
3.7
作者：
Hawe, Andrea;Sutter, Marc;Jiskoot, Wim
通讯作者：
Jiskoot, Wim
影响因子：
7.4
作者：
Chen, QH;Thorpe, J;Keller, JN
通讯作者：
Keller, JN
DOI：
10.1073/pnas.1332607100
发表时间：
2003-06-24
影响因子：
11.1
作者：
Moskovitz, J;Stadtman, ER
通讯作者：
Stadtman, ER
影响因子：
3.1
作者：
Brandis, KA;Holmes, IF;DebBurman, SK
通讯作者：
DebBurman, SK
影响因子：
4.8
作者：
Chen, QH;Thorpe, J;Keller, JN
通讯作者：
Keller, JN