Extrinsic fluorescent dyes as tools for protein characterization.
Extrinsic fluorescent dyes as tools for protein characterization.
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DOI：
10.1007/s11095-007-9516-9
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发表时间：
2008-07
影响因子：
3.7
通讯作者：
Jiskoot, Wim
中科院分区：
文献类型：
--
作者：
Hawe, Andrea;Sutter, Marc;Jiskoot, Wim
关键词：
非共价、外源性荧光染料被应用于蛋白质分析的各个领域，例如，表征折叠中间体，测量表面疏水性，检测聚集或纤颤。解释许多外来染料荧光特性的主要潜在机制是溶剂弛豫过程和（扭曲的）分子内电荷转移反应，这些反应受环境和染料与蛋白质的相互作用的影响。近年来，外源性荧光染料如ANS、bisans、Nile Red、thiioflavin T等因其通用性强、灵敏度高、适合高通量筛选而得到越来越多的应用。本文综述了现有的外源染料，解释了它们的光谱特性，并举例说明了它们在蛋白质表征中的各种应用。
Noncovalent, extrinsic fluorescent dyes are applied in various fields of protein analysis, e.g. to characterize folding intermediates, measure surface hydrophobicity, and detect aggregation or fibrillation. The main underlying mechanisms, which explain the fluorescence properties of many extrinsic dyes, are solvent relaxation processes and (twisted) intramolecular charge transfer reactions, which are affected by the environment and by interactions of the dyes with proteins. In recent time, the use of extrinsic fluorescent dyes such as ANS, Bis-ANS, Nile Red, Thioflavin T and others has increased, because of their versatility, sensitivity and suitability for high-throughput screening. The intention of this review is to give an overview of available extrinsic dyes, explain their spectral properties, and show illustrative examples of their various applications in protein characterization.
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