Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate.
Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate.
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DOI：
10.1186/1471-2164-8-190
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发表时间：
2007-06-25
期刊：
影响因子：
4.4
通讯作者：
Fuchs E
中科院分区：
文献类型：
--
作者：
Datson NA;Morsink MC;Atanasova S;Armstrong VW;Zischler H;Schlumbohm C;Dutilh BE;Huynen MA;Waegele B;Ruepp A;de Kloet ER;Fuchs E
普通狨猴 (Callithrix jacchus) 是一种原产于巴西东部的小型非濒危新大陆灵长类动物，在生物医学研究、药物开发和安全评估中越来越多地用作非人类灵长类动物模型。与狨猴作为动物模型日益增长的兴趣相比，用于遗传分析的分子工具极其有限。在这里，我们报告了第一个狨猴特异性寡核苷酸微阵列（EUMAMA）的开发，该微阵列包含针对海马中表达的 1541 种不同狨猴转录物的探针组。这 1541 个转录本代表了多种不同的功能基因类别。将狨猴微阵列与来自海马体、皮质和一组 7 种不同外周组织的标记 RNA 进行杂交，神经元组织的检出率高达 85%，非神经元组织的检出率平均为 70%。海马和皮层这两种神经元组织的表达谱高度相似，相关系数为0.96。鉴定了几个具有组织特异性表达模式的转录本。除了狨猴微阵列之外，我们还生成了来自狨猴海马的 3215 个 EST，这些 EST 已注释并提交给 GenBank [GenBank：EF214838 – EF215447、EH380242 – EH382846]。我们已经生成了第一个狨猴特异性 DNA 微阵列，并证明了其可用于表征海马以及其他神经元和非神经元组织的大规模基因表达谱。此外，我们还生成了大量狨猴起源的 EST，现已在公共领域提供。这些新工具将促进对这种非人类灵长类动物模型的分子遗传学研究。
The common marmoset monkey (Callithrix jacchus), a small non-endangered New World primate native to eastern Brazil, is becoming increasingly used as a non-human primate model in biomedical research, drug development and safety assessment. In contrast to the growing interest for the marmoset as an animal model, the molecular tools for genetic analysis are extremely limited. Here we report the development of the first marmoset-specific oligonucleotide microarray (EUMAMA) containing probe sets targeting 1541 different marmoset transcripts expressed in hippocampus. These 1541 transcripts represent a wide variety of different functional gene classes. Hybridisation of the marmoset microarray with labelled RNA from hippocampus, cortex and a panel of 7 different peripheral tissues resulted in high detection rates of 85% in the neuronal tissues and on average 70% in the non-neuronal tissues. The expression profiles of the 2 neuronal tissues, hippocampus and cortex, were highly similar, as indicated by a correlation coefficient of 0.96. Several transcripts with a tissue-specific pattern of expression were identified. Besides the marmoset microarray we have generated 3215 ESTs derived from marmoset hippocampus, which have been annotated and submitted to GenBank [GenBank: EF214838 – EF215447, EH380242 – EH382846]. We have generated the first marmoset-specific DNA microarray and demonstrated its use to characterise large-scale gene expression profiles of hippocampus but also of other neuronal and non-neuronal tissues. In addition, we have generated a large collection of ESTs of marmoset origin, which are now available in the public domain. These new tools will facilitate molecular genetic research into this non-human primate animal model.
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