Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing.
Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing.
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DOI：
10.1016/j.snb.2014.10.107
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发表时间：
2015-02-01
影响因子：
8.4
通讯作者：
Ma, Yinfa
中科院分区：
文献类型：
--
作者：
Yang, Qingbo;Wang, Hanzheng;Lan, Xinwei;Cheng, Baokai;Chen, Sisi;Shi, Honglan;Xiao, Hai;Ma, Yinfa
关键词：
在单细胞水平上进行pH传感而不对活细胞产生负面影响是非常重要的，但仍然是生物医学研究中的一个遗留问题。设计并制作了一种70 μm的反射式光纤微pH传感器。采用pH敏感荧光染料2′，7′-双（2-羰基乙基）-5（6）-羧基荧光素（BCECF）共价键合在气凝胶网络中。通过调节醇盐的混合比例和六甲基二硅氮烷（HMDS）的引发程序，可以优化传感器，使其具有高稳定性和pH传感能力。然后以简单的光谱方式证明了体外实时传感能力，并且在6.12-7.81的狭窄但具有生物学意义的pH范围内显示出pH分辨率高达平均0.049 pH单位的线性测量响应。它的高空间分辨率，反射模式操作，快速响应和高稳定性，在生物有意义的pH范围内的大的线性响应和高pH分辨率的新特性，使这种新型pH探针非常具有成本效益的工具，用于化学/生物传感，特别是在单细胞水平的研究领域。
pH sensing at the single-cell level without negatively affecting living cells is very important but still a remaining issue in the biomedical studies. A 70 μm reflection-mode fiber-optic micro-pH sensor was designed and fabricated by dip-coating thin layer of organically modified aerogel onto a tapered spherical probe head. A pH sensitive fluorescent dye 2′, 7′-Bis (2-carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was employed and covalently bonded within the aerogel networks. By tuning the alkoxide mixing ratio and adjusting hexamethyldisilazane (HMDS) priming procedure, the sensor can be optimized to have high stability and pH sensing ability. The in vitro real-time sensing capability was then demonstrated in a simple spectroscopic way, and showed linear measurement responses with a pH resolution up to an average of 0.049 pH unit within a narrow, but biological meaningful pH range of 6.12–7.81. Its novel characterizations of high spatial resolution, reflection mode operation, fast response and high stability, great linear response within biological meaningful pH range and high pH resolutions, make this novel pH probe a very cost-effective tool for chemical/biological sensing, especially within the single cell level research field.
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影响因子：
8.4
作者：
Guo, Liangqia;Zhang, Wei;Chen, Guonan
通讯作者：
Chen, Guonan
影响因子：
4.2
作者：
Higgins, Clare;Wencel, Dorota;McDonagh, Colette
通讯作者：
McDonagh, Colette
影响因子：
8.6
作者：
Menaa, Bouzid;Takahashi, Masahide;Yoko, Toshinobu
通讯作者：
Yoko, Toshinobu
影响因子：
3.5
作者：
Bukowski, Rachel M.;Davenport, Michael D.;Bright, Frank V.
通讯作者：
Bright, Frank V.
DOI：
10.1080/03067310902903738
发表时间：
2010-01-01
影响因子：
2.6
作者：
El-Nahhal, Issa Mussa;Zourab, Shehata Mahmoud;Al-Bawab, Abeer
通讯作者：
Al-Bawab, Abeer